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Determination of glycated albumin using a Prussian blue nanozyme-based boronate affinity sandwich assay

Title
Determination of glycated albumin using a Prussian blue nanozyme-based boronate affinity sandwich assay
Author
성기훈
Issue Date
2020-10
Publisher
ELSEVIER
Citation
ANALYTICA CHIMICA ACTA, v. 1134, Page. 41-49
Abstract
Nanozymes are effective substitutes for natural enzymes and offer multiple advantages. Here, synthesized Prussian blue nanoparticles (PBNPs) exhibited excellent peroxidase-like activity, catalyzing the oxidation of 3,5,3',5'-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide within 1 min. Oxidized TMB (TMBox) underwent a color change from transparent to blue and then yellow by a stop solution. Moreover, the TMBox could be reduced on an indium tin oxide electrode, generating an electrochemical current, indicating that TMB can be used as a colorimetric and electrochemical indicator. The PBNPs modified with 3-aminophenylboronic acid (APBA) captured glycated albumin (GA) with a boronate affinity sandwich assay. As boronic acid binds to glycoproteins using cis-diol bonding, it can be used to detect GA. The APBA-modified PBNPs (PBBA) were involved with a sandwich complex formation and employed as nanozymes for the quantitative analysis of GA using colorimetric and electrochemical methods. Both methods showed strong linearities for different concentrations of GA. The results show that PBBA is a suitable alternative for natural enzymes and can be applied to sensitive determination of GA. (C) 2020 Elsevier B.V. All rights reserved.
URI
https://www.sciencedirect.com/science/article/pii/S0003267020308461https://repository.hanyang.ac.kr/handle/20.500.11754/165340
ISSN
0003-2670
DOI
10.1016/j.aca.2020.08.015
Appears in Collections:
COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > BIONANO ENGINEERING(생명나노공학과) > Articles
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