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One-pot synthesized citric acid-modified bimetallic PtNi hollow nanospheres as peroxidase mimics for colorimetric detection of human serum albumin

Title
One-pot synthesized citric acid-modified bimetallic PtNi hollow nanospheres as peroxidase mimics for colorimetric detection of human serum albumin
Author
성기훈
Keywords
Noble metal; Bimetallic; Hollow nanospheres; Nanozymes; Peroxidase-like activity
Issue Date
2020-11
Publisher
ELSEVIER
Citation
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, v. 116, Article no. 111231, 11pp
Abstract
The combination of Pt with low-cost transition metal is an effective way to diminish the bulk utilization of costly Pt and to design new nanostructured materials with improved enzyme-like activity. In the present work, citric acid-functionalized platinum-nickel hollow nanospheres (CA@PtNi hNS) were synthesized through a simple one-pot wet chemical method, which involves the galvanic replacement reaction between the Ni nanoparticles and the Pt precursor that leads to the formation of hollow nanostructures. Transmission electron spectroscopic images revealed the uniformity of the CA@PtNi hNS, with an average diameter of 10.3 +/- 2 nm. Moreover, zeta potential, FTIR, and XPS measurements confirmed the existence of citric acid in the CA@PtNi hNS. During synthesis, the use of citric acid not only facilitates monodispersity but also provides a negative surface charge (-11 mV) to the CA@PtNi hNS that electrostatically attracts the 3,3',5,5'-Tetramethylbenzidine (TMB) substrate. As-prepared CA@PtNi hNS possessed excellent peroxidase-like activity due to rich Pt surfaces, large surface area, and heterogeneous interaction between Pt and Ni atoms. Furthermore, a nanozyme-linked immunosorbent assay (NLISA) for human serum albumin (HSA) detection was developed by replacing the enzyme in a standard enzyme-linked immunosorbent assay with CA@PtNi hNS. The CA@PtNi hNS based-NLISA showed sensitive detection of HSA concentrations ranging from 0 to 400 ng mL(-1) with a LOD of 0.19 ng mL(-1) and an average of 112% recovery of HSA from the spiked human plasma samples. The outcomes of the present study confirm the applicability of CA@PtNi hNS as substitutes for natural enzymes.
URI
https://www.sciencedirect.com/science/article/pii/S0928493120308821https://repository.hanyang.ac.kr/handle/20.500.11754/165251
ISSN
0928-4931
DOI
10.1016/j.msec.2020.111231
Appears in Collections:
COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > BIONANO ENGINEERING(생명나노공학과) > Articles
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