Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 허준호 | - |
dc.date.accessioned | 2021-09-27T08:09:48Z | - |
dc.date.available | 2021-09-27T08:09:48Z | - |
dc.date.issued | 2020-03 | - |
dc.identifier.citation | TRENDS IN MOLECULAR MEDICINE, v. 26, no. 3 page. 337-350 | en_US |
dc.identifier.issn | 1471-4914 | - |
dc.identifier.issn | 1471-499X | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S147149141930262X?via%3Dihub | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/165168 | - |
dc.description.abstract | Clustered regularly interspaced short palindromic repeats, or CRISPR, has been widely accepted as a versatile genome editing tool with significant potential for medical application. Reliable allele specificity is one of the most critical elements for successful application of this technology to develop high-precision therapeutics and diagnostics. CRISPR-based genome editing tools achieve high-fidelity distinction of single-base differences in target genomic loci by structural identification of CRISPR-associated (Cas) proteins and sequences of the guide RNAs. In this review, we describe the structural features of ribonucleoprotein complex formation by CRISPR proteins and guide RNAs that eventually recognize target DNA sequences. This structural understanding provides the basis for the recent applications of enhanced single-base precision genome editing technologies for effective distinction of specific alleles. | en_US |
dc.description.sponsorship | This research was supported by grants from the National Research Foundation (NRF) funded by the Korean Ministry of Education, Science and Technology (NRF-2019R1C1C1006603 and NRF-2018M3A9H1023142 to S.H. L., and NRF-2017R1E1A1A01074529, NRF-2018M3A9H3021707 to J.K.H.). The study was also supported by the grants from the Korea Research Institute of Bioscience & Biotechnology (KRIBB; Research Initiative Program KGM4251824 and KGM5381911 to S.H.L.). | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER SCI LTD | en_US |
dc.subject | GENOME-WIDE SPECIFICITIES | en_US |
dc.subject | RNA-GUIDED ENDONUCLEASE | en_US |
dc.subject | R-LOOP COMPLEX | en_US |
dc.subject | IN-VITRO | en_US |
dc.subject | CRYSTAL-STRUCTURE | en_US |
dc.subject | HUMAN-CELLS | en_US |
dc.subject | DUAL-RNA | en_US |
dc.subject | DNA | en_US |
dc.subject | CAS9 | en_US |
dc.subject | MUTATIONS | en_US |
dc.title | CRISPR Diagnosis and Therapeutics with Single Base Pair Precision | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1016/j.molmed.2019.09.008 | - |
dc.relation.journal | TRENDS IN MOLECULAR MEDICINE | - |
dc.contributor.googleauthor | Lee, Seung Hwan | - |
dc.contributor.googleauthor | Park, Young-Ho | - |
dc.contributor.googleauthor | Jin, Yeung Bae | - |
dc.contributor.googleauthor | Kim, Sun-Uk | - |
dc.contributor.googleauthor | Hur, Junho K. | - |
dc.relation.code | 2020046567 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | juhur | - |
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