Bioassay-Guided Separation ofCentipeda minimaUsing Comprehensive Linear Gradient Centrifugal Partition Chromatography
- Title
- Bioassay-Guided Separation ofCentipeda minimaUsing Comprehensive Linear Gradient Centrifugal Partition Chromatography
- Author
- 김철영
- Keywords
- centrifugal partition chromatography; comprehensive linear gradient elution; bioactivity-guided isolation; 3-methoxyquercetin; brevilin A; Centipeda minima
- Issue Date
- 2020-07
- Publisher
- MDPI
- Citation
- MOLECULES, v. 25, Issue. 13, Article no. 3077, 10pp
- Abstract
- A comprehensive linear gradient solvent system for centrifugal partition chromatography (CPC) was developed for the bioassay-guided isolation of natural compounds. The gradient solvent system consisted of three different ternary biphasic solvents types:n-hexane-acetonitrile-water (10:2:8,v/v), ethyl acetate-acetonitrile-water (10:2:8,v/v), and water-saturatedn-butanol-acetonitrile-water (10:2:8,v/v). The lower phase of then-hexane-acetonitrile-water (10:2:8,v/v) was used as the stationary phase, while its upper phase, as well as ethyl acetate-acetonitrile-water (10:2:8), and water-saturatedn-butanol-acetonitrile-water (10:2:8,v/v) were pumped to generate a linear gradient elution, increasing the mobile phase polarity. We used the gradient CPC to identify antioxidant response elements (AREs), inducing compounds fromCentipeda minima, using an ARE-luciferase assay in HepG2 cells, which led to the purification of the active molecules 3-methoxyquercetin and brevilin A. The developed CPC solvent systems allow the separation and isolation of compounds with a wide polarity range, allowing active molecule identification in the complex crude extract of natural products.
- URI
- https://www.mdpi.com/1420-3049/25/13/3077https://repository.hanyang.ac.kr/handle/20.500.11754/164861
- ISSN
- 1420-3049
- DOI
- 10.3390/molecules25133077
- Appears in Collections:
- COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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