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Effects of ethanol preservation and formalin fixation on amino acid stable isotope analysis (delta C-13 and delta N-15) and its ecological applications

Title
Effects of ethanol preservation and formalin fixation on amino acid stable isotope analysis (delta C-13 and delta N-15) and its ecological applications
Author
신경훈
Issue Date
2020-03
Publisher
WILEY
Citation
LIMNOLOGY AND OCEANOGRAPHY-METHODS, v. 18, no. 2, page. 77-88
Abstract
Compound-specific isotope analysis of amino acids (CSIA-AA) is a promising nascent technique that alleviates many shortcomings of conventional bulk-tissue stable isotope analysis ("bulk SIA") in ecological studies involving the tracing/reconstruction of carbon and nitrogen pathways. While CSIA-AA has been increasingly applied to preserved tissue samples (e.g., material in natural history collections), the effects of sample preservation on amino acid delta C-13 and delta N-15 profiles are poorly understood. It is therefore unclear if mathematical correction factors are necessary for interpreting isotopic profiles of preserved samples. In this study, we investigated effects of ethanol preservation and formalin fixation on amino acid delta C-13 and delta N-15 profiles. We also assess how these effects translate to two ecological applications of CSIA-AA: quantification of organic carbon sources, and estimation of trophic positions. Results from an 8-week controlled experiment on freshwater fish tissue show negligible preservation effects on most amino acid delta N-15 profiles, and results are similar for delta C-13 profiles of essential amino acids. Findings from mixing models using essential amino acid delta C-13 profiles similarly show that preserved samples can yield robust estimates of carbon source contributions. We also empirically demonstrate, for the first time, the use of amino acid delta C-13 profiles to enhance delta N-15-based estimates of trophic position in food webs with multiple producers, and show that these estimates are not compromised by preservation effects. Overall, our findings support the view that amino acid delta C-13 and delta N-15 profiles from ethanol- and formalin-treated CSIA-AA samples can be directly used for addressing ecological questions.
URI
https://aslopubs.onlinelibrary.wiley.com/doi/full/10.1002/lom3.10347https://repository.hanyang.ac.kr/handle/20.500.11754/163095
ISSN
1541-5856
DOI
10.1002/lom3.10347
Appears in Collections:
COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > MARINE SCIENCE AND CONVERGENCE ENGINEERING(해양융합공학과) > Articles
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