189 0

Full metadata record

DC FieldValueLanguage
dc.contributor.author이영한-
dc.date.accessioned2021-07-22T05:02:53Z-
dc.date.available2021-07-22T05:02:53Z-
dc.date.issued2000-07-
dc.identifier.citationJournal of Neurochemistry, v. 75, issue. 1, page. 274-281en_US
dc.identifier.issn0022-3042-
dc.identifier.issn1471-4159-
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/full/10.1046/j.1471-4159.2000.0750274.x-
dc.identifier.urihttps://repository.hanyang.ac.kr/handle/20.500.11754/163019-
dc.description.abstractRecently, we have isolated a cDNA encoding a muscarinic acetylcholine receptor (mAChR) from Caenorhabditis elegans. To investigate the regulation of phospholipase D (PLD) signaling via a muscarinic receptor, we generated stable transfected Chinese hamster ovary (CHO) cells that overexpress the mAChR of C. elegans (CHO‐GAR‐3). Carbachol (CCh) induced inositol phosphate formation and a significantly higher Ca2+ elevation and stimulated PLD activity through the mAChR ; this was insensitive to pertussis toxin, but its activity was abolished by the phospholipase C (PLC) inhibitor U73122. Western blot analysis revealed several apparent tyrosine‐phosphorylated protein bands after CCh treatment. The CCh‐induced PLD activation and tyrosine phosphorylation were significantly reduced by the protein kinase C (PKC) inhibitor calphostin C and down‐regulation of PKC and the tyrosine kinase inhibitor genistein. Moreover, the Ca2+‐calmodulin‐dependent protein kinase II (CaM kinase II) inhibitor KN62, in addition to chelation of extracellular or intracellular Ca2+ by EGTA and BAPTA/AM, abolished CCh‐induced PLD activation and protein tyrosine phosphorylation. Taken together, these results suggest that the PLC/PKC‐PLD pathway and the CaM kinase II/tyrosine kinase‐PLD pathway are involved in the activation of PLD through mAChRs of C. elegans.en_US
dc.language.isoen_USen_US
dc.publisherWILEYen_US
dc.titlePhospholipase C, protein kinase C and Ca2+/calmodulin-dependent protein kinase II are involved in carbachol-induced phospholipase D activation in Chinese hamster ovary cells expressing muscarinic acetylcholine receptor of Caenorhabditid elegans.en_US
dc.typeArticleen_US
dc.identifier.doi10.1046/j.1471-4159.2000.0750274.x-
dc.relation.journalJOURNAL OF NEUROCHEMISTRY-
dc.contributor.googleauthorMin, Do Sik-
dc.contributor.googleauthorCho, Nam Jeong-
dc.contributor.googleauthorYoon, Shin Hee-
dc.contributor.googleauthorHahn, Sang‐June-
dc.contributor.googleauthorLee, Kweon‐Haeng-
dc.contributor.googleauthorKim, Myung‐Suk-
dc.contributor.googleauthorJo, Yang‐Hyeok-
dc.contributor.googleauthorLee, Young Han-
dc.relation.code2009205492-
dc.sector.campusE-
dc.sector.daehakCOLLEGE OF SCIENCE & TECHNOLOGY[E]-
dc.sector.departmentDIVISION OF MOLECULAR & LIFE SCIENCE-
dc.identifier.pidyounghan-
Appears in Collections:
COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > ETC
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE