Overexpression of phospholipase C-β1 protects NIH3T3 cells from oxidative stress-induced cell death
- Title
- Overexpression of phospholipase C-β1 protects NIH3T3 cells from oxidative stress-induced cell death
- Author
- 이영한
- Issue Date
- 2000-07
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Citation
- LIFE SCIENCES, v. 67, issue. 7, page. 827-837
- Abstract
- Oxidative stress has been implicated in a wide range of cellular damage which includes DNA oxidation, membrane lipid peroxidation, and apoptosis. In our study, we found that overexpression of PLC-β1 in NIH3T3 fibroblasts protected them from cell death occuring in response to oxidative stress. Cell death caused by treatment with prooxidant tert-butylhydroperoxide (TBH), H2O2, or CdCl2 was considerably suppressed in PLC-β1 overexpressed NIH/β1-14 cells in comparison to control NIH/neo cells. However, overexpression of PLC-β1 failed to protect the cells from toxicity by diamide or KCN. In addition, while accumulation of c-fos mRNA was observed within 30 min of TBH treatment in vector transfected NIH/neo cells, TBH-induced c-fos mRNA generation was completely suppressed in NIH/β1-14 cells, while that of c-jun and GAPDH was not affected. These findings suggest that PLC-β1 may play a role in process that can protect cells from oxidative stress-induced cell death.
- URI
- https://www.sciencedirect.com/science/article/pii/S0024320500006779https://repository.hanyang.ac.kr/handle/20.500.11754/162988
- ISSN
- 0024-3205
- DOI
- 10.1016/S0024-3205(00)00677-9
- Appears in Collections:
- COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > ETC
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