Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이영한 | - |
dc.date.accessioned | 2021-03-31T04:13:58Z | - |
dc.date.available | 2021-03-31T04:13:58Z | - |
dc.date.issued | 2001-11 | - |
dc.identifier.citation | ONCOGENE, v.20, issue. 55, page. 7954-7964 | en_US |
dc.identifier.issn | 0950-9232 | - |
dc.identifier.uri | http://eds.a.ebscohost.com/eds/detail/detail?vid=0&sid=0eebf345-3bde-4dad-b45e-a874db2bf5f9%40sdc-v-sessmgr02&bdata=Jmxhbmc9a28mc2l0ZT1lZHMtbGl2ZQ%3d%3d#db=a9h&AN=8919746 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/161024 | - |
dc.description.abstract | Phospholipase C-γ1(PLC-γ1) is known to play an essential role in various cellular responses, such as proliferation and tumorigenesis, and PLC-γ1-specific inhibitors are commonly employed to investigate the mechanism of the PLC-γ1-mediated signaling pathway. In this study, we developed a single chain antibody fragment (scFv) as a blocker for PLC-γ1 mediated signaling. scFv, designated F7-scFv, specifically bound to PLC-γ1 with high affinity (Kd=1.9×10-8 M) in vitro. F7-scFv also bound to PLC-γ1 in vivo and altered the distribution pattern of PLC-γ1 from the cytoplasm to the intracellular aggregates, where F7-scFv was localized. Moreover, F7-scFv interrupted the EGF-induced translocation of PLC-γ1 from the cytosol to the membrane ruffle and attenuated EGF-induced inositol phosphates generation and intracellular calcium mobilization. These results indicate that F7-scFv blocks EGF-induced PLC-γ1 activation by causing sequestering of PLC-γ1 into intracellular aggregates, and may therefore be useful in studies of the PLC-γ1-mediated signaling pathway. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | NATURE PUBLISHING GROUP | en_US |
dc.subject | PLC-g1 | en_US |
dc.subject | single chain antibody (scFv) | en_US |
dc.subject | EGF | en_US |
dc.title | Inhibition of the EGF-induced activation of phospholipase C-γ1 by a single chain antibody fragment. | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1038/sj.onc.1204959 | - |
dc.relation.journal | ONCOGENE | - |
dc.contributor.googleauthor | Yi, Kye Sook | - |
dc.contributor.googleauthor | Chung, Jun Ho | - |
dc.contributor.googleauthor | Chung, Hyang Gi | - |
dc.contributor.googleauthor | Kim, Ik Jung | - |
dc.contributor.googleauthor | Suh, Byung Chang | - |
dc.contributor.googleauthor | Kim, Euikyung | - |
dc.contributor.googleauthor | Cocco, Lucio | - |
dc.contributor.googleauthor | Ryu, Sung Ho | - |
dc.contributor.googleauthor | Suh, Pann-Ghill | - |
dc.contributor.googleauthor | Lee, Young Han | - |
dc.relation.code | 2009207260 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF SCIENCE & TECHNOLOGY[E] | - |
dc.sector.department | DIVISION OF MOLECULAR & LIFE SCIENCE | - |
dc.identifier.pid | younghan | - |
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