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Inhibition of the EGF-induced activation of phospholipase C-γ1 by a single chain antibody fragment.

Title
Inhibition of the EGF-induced activation of phospholipase C-γ1 by a single chain antibody fragment.
Author
이영한
Keywords
PLC-g1; single chain antibody (scFv); EGF
Issue Date
2001-11
Publisher
NATURE PUBLISHING GROUP
Citation
ONCOGENE, v.20, issue. 55, page. 7954-7964
Abstract
Phospholipase C-γ1(PLC-γ1) is known to play an essential role in various cellular responses, such as proliferation and tumorigenesis, and PLC-γ1-specific inhibitors are commonly employed to investigate the mechanism of the PLC-γ1-mediated signaling pathway. In this study, we developed a single chain antibody fragment (scFv) as a blocker for PLC-γ1 mediated signaling. scFv, designated F7-scFv, specifically bound to PLC-γ1 with high affinity (Kd=1.9×10-8 M) in vitro. F7-scFv also bound to PLC-γ1 in vivo and altered the distribution pattern of PLC-γ1 from the cytoplasm to the intracellular aggregates, where F7-scFv was localized. Moreover, F7-scFv interrupted the EGF-induced translocation of PLC-γ1 from the cytosol to the membrane ruffle and attenuated EGF-induced inositol phosphates generation and intracellular calcium mobilization. These results indicate that F7-scFv blocks EGF-induced PLC-γ1 activation by causing sequestering of PLC-γ1 into intracellular aggregates, and may therefore be useful in studies of the PLC-γ1-mediated signaling pathway.
URI
http://eds.a.ebscohost.com/eds/detail/detail?vid=0&sid=0eebf345-3bde-4dad-b45e-a874db2bf5f9%40sdc-v-sessmgr02&bdata=Jmxhbmc9a28mc2l0ZT1lZHMtbGl2ZQ%3d%3d#db=a9h&AN=8919746https://repository.hanyang.ac.kr/handle/20.500.11754/161024
ISSN
0950-9232
DOI
10.1038/sj.onc.1204959
Appears in Collections:
COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > ETC
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