Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 정희용 | - |
dc.contributor.author | 이주연 | - |
dc.date.accessioned | 2021-02-24T16:08:22Z | - |
dc.date.available | 2021-02-24T16:08:22Z | - |
dc.date.issued | 2021. 2 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/159085 | - |
dc.identifier.uri | http://hanyang.dcollection.net/common/orgView/200000485708 | en_US |
dc.description.abstract | Oncogenic RAS mutations are critical in inducing several forms of human myeloid leukemia. Previous study has shown that NRASG12D alone is not sufficient to promote malignant tumors. Therefore, I screened several oncogenes (C/EBPα p30, IRX3, and CBFβ-MYH11) that have been known to affect myeloid differentiation for collaboration with NRASG12D in leukemia induction. I confirmed synergistic effect of C/EBPα p30 and NRASG12D combination in in vitro proliferation assay. The transcriptions factor CEBPA (CCAAT/enhancer binding protein α) is crucial for myeloid differentiation and growth arrest. Mutations in the CEBPA genes are found in about 10% of acute myeloid leukemia (AML) patients, and they are mostly biallelic CEBPA mutations. One mutation leads to the frame-shifted mutation at N-terminus (C/EBPα p30) and the other leads to C-terminal point mutations at the basic leucine zipper domain (C/EBPα BRM). In this study, I investigated three forms of C/EBPα mutations and their combinations for their ability of inducing leukemia in collaboration with NRASG12D. The three C/EBPα mutant forms used in this experiment are p30, BRM2 and dual mutation of p30 and BRM. Among these C/EBPα mutants, p30-BRM dual mutant form, having both C-terminal and N-terminal mutation in a single cistron, showed most potent synergistic effect with NRASG12D. With this system, I analyzed the effect of BRM2 mutation in shifting the transcriptome of leukemic cells in the presence of NRASG12D and I believe the results from this study will offer unique opportunity of understanding the mechanism of the appearance of biallelic C/EBPα mutation in myeloid leukemia patients. | - |
dc.publisher | 한양대학교 | - |
dc.title | Role of CEBPA mutations in RAS-mediated leukemogenesis | - |
dc.type | Theses | - |
dc.contributor.googleauthor | Ju Yeon Lee | - |
dc.contributor.alternativeauthor | 이주연 | - |
dc.sector.campus | S | - |
dc.sector.daehak | 의생명공학전문대학원 | - |
dc.sector.department | 의생명과학과 | - |
dc.description.degree | Master | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.