Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이민형 | - |
dc.date.accessioned | 2021-02-17T02:14:59Z | - |
dc.date.available | 2021-02-17T02:14:59Z | - |
dc.date.issued | 2019-12 | - |
dc.identifier.citation | JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, v. 15, no. 12, page. 2401-2412 | en_US |
dc.identifier.issn | 1550-7033 | - |
dc.identifier.issn | 1550-7041 | - |
dc.identifier.uri | https://www.ingentaconnect.com/content/asp/jbn/2019/00000015/00000012/art00009 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/158531 | - |
dc.description.abstract | Ischemic strokes are caused by decreased blood flow into the brain, due to narrowed cerebral arteries. In the ischemic brain, high-mobility group box 1 (HMGB1) is released into extracellular spaces and induces inflammatory reactions. In this study, HMGB1 small interfering RNA (siRNA) was delivered into ischemic brains by intravenous administration using rabies virus glycoprotein (RVG) peptide-decorated exosomes. A fusion protein of RVG and Lamp2b was expressed in 293T cells. Since Lamp2b is an exosome membrane-integral protein, RVG-Lamp2b is integrated into the exosomes, producing RVG-decorated exosomes (RVG-Exo). HMGB1-siRNA was loaded into RVG-Exo and unmodified exosomes (Unmod-Exo) by electroporation. The exosomes were homogenous with a size of less than 50 nm and a negative surface charge. In vitro delivery assays showed that RVG-Exo showed higher efficiency to Neuro2A cells than Unmod-Exo. Also, HMGB1 levels were reduced more effectively by RVG-Exo/HMGB1-siRNA. In vivo delivery efficiency and therapeutic effects of RVG-Exo/HMGB1-siRNA were evaluated in a middle cerebral artery occlusion (MCAO) model. RVG-Exo/HMGB1-siRNA, Unmod-Exo/HMGB1-siRNA, and PEI25k/HMGB1-siRNA were administrated into the MCAO model intravenously through the tail vein. The results showed that HMGB1, tumor necrosis factor-alpha (TNF-alpha), and apoptosis levels in the brain were reduced in the RVG-Exo/HMGB1-siRNA group more efficiently than the other groups. In addition, the infarct size was decreased in the RVG-Exo/HMGB1 group more effectively than the other groups. These results suggest that RVG-Exo with HMGB1-siRNA may have potential as a therapeutic system for the treatment of ischemic strokes. | en_US |
dc.description.sponsorship | This work was supported by the Individual Basic Science & Engineering Research Program (NRF-2017R1A2B4009036) and Bio & Medical Technology Development Program (NRF-2016M3A9B4918833) through the National Research Foundation funded by the Ministry of Science and ICT. | en_US |
dc.language.iso | en | en_US |
dc.publisher | AMER SCIENTIFIC PUBLISHERS | en_US |
dc.subject | Exosome | en_US |
dc.subject | Rabies Virus Glycoprotein (RVG) Peptide | en_US |
dc.subject | High Mobility Group Box 1 (HMGB1) | en_US |
dc.subject | Small Interfering RNA (siRNA) | en_US |
dc.subject | Ischemic Stroke | en_US |
dc.title | Delivery of High Mobility Group Box-1 siRNA Using Brain-Targeting Exosomes for Ischemic Stroke Therapy | en_US |
dc.type | Article | en_US |
dc.relation.no | 12 | - |
dc.relation.volume | 15 | - |
dc.identifier.doi | 10.1166/jbn.2019.2866 | - |
dc.relation.page | 2401-2412 | - |
dc.relation.journal | JOURNAL OF BIOMEDICAL NANOTECHNOLOGY | - |
dc.contributor.googleauthor | Kim, Minkyung | - |
dc.contributor.googleauthor | Kim, Gyeungyun | - |
dc.contributor.googleauthor | Hwang, Do Won | - |
dc.contributor.googleauthor | Lee, Minhyung | - |
dc.relation.code | 2019002565 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | minhyung | - |
dc.identifier.researcherID | W-1245-2018 | - |
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