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dc.contributor.author이영한-
dc.date.accessioned2021-02-09T04:51:43Z-
dc.date.available2021-02-09T04:51:43Z-
dc.date.issued2002-11-
dc.identifier.citationEXPERIMENTAL CELL RESEARCH, v. 280, issue. 2, page. 280-287en_US
dc.identifier.issn0014-4827-
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0014482702956491-
dc.identifier.urihttps://repository.hanyang.ac.kr/handle/20.500.11754/157981-
dc.description.abstractTo identify genes involved in cervical carcinogenesis, the mRNA differential display method was used. A 220-bp cDNA fragment called CA11 was present in normal cervical tissue but not in primary cervical cancer tissue or cervical cancer cell lines. CA11 exhibited 98% homology with the recorded human secreted frizzled-related protein (hsFRP) sequence. A dominant hsFRP mRNA transcript of approximately 4.6 kb was present in three normal cervical tissues examined. Expression of the transcript was nearly absent from three cervical cancer tissues and from five human cervical cancer-derived cell lines. Results from in situ hybridization showed that the hsFRP transcript was confined to the normal cervical epithelial layer. When hsFRP-transfected HeLa and CUMC-6 cervical cancer cells were cultured in serum-free medium, most of the cells died within 8 days. This effect is associated with the apoptotic process. The caspase-3 inhibitor 1, Ac-DEVD-CHO, blocked hsFRP-induced apoptotic cell death. Additionally, cleavage of poly (ADP-ribose) polymerase in hsFRP-transfected cells was confirmed by colorimetric assay. These results indicate that the hsFRP gene probably functions as a tumor suppressor in normal cervical epithelium and down-regulation of hsFRP contributes to development of cervical cancer.en_US
dc.language.isoen_USen_US
dc.publisherElsevier Inc.en_US
dc.subjecthuman secreted frizzled-related proteinen_US
dc.subjectcervical canceren_US
dc.subjecttumor suppressoren_US
dc.subjectβ-cateninen_US
dc.subjectapoptosisen_US
dc.titleHuman secreted frizzled-related protein is down-regulated and induces apoptosis in human cervical cancer.en_US
dc.typeArticleen_US
dc.identifier.doi10.1006/excr.2002.5649-
dc.relation.journalEXPERIMENTAL CELL RESEARCH-
dc.contributor.googleauthorKo, Jesang-
dc.contributor.googleauthorRyu, Ki Sung-
dc.contributor.googleauthorLee, Young Han-
dc.contributor.googleauthorNa, Doe Sun-
dc.contributor.googleauthorKim, Yoon Suk-
dc.contributor.googleauthorOh, Young Mi-
dc.contributor.googleauthorKim, In Sik-
dc.contributor.googleauthorKim, Jin Woo-
dc.relation.code2009203131-
dc.sector.campusE-
dc.sector.daehakCOLLEGE OF SCIENCE & TECHNOLOGY[E]-
dc.sector.departmentDIVISION OF MOLECULAR & LIFE SCIENCE-
dc.identifier.pidyounghan-
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COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > ETC
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