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Human secreted frizzled-related protein is down-regulated and induces apoptosis in human cervical cancer.

Title
Human secreted frizzled-related protein is down-regulated and induces apoptosis in human cervical cancer.
Author
이영한
Keywords
human secreted frizzled-related protein; cervical cancer; tumor suppressor; β-catenin; apoptosis
Issue Date
2002-11
Publisher
Elsevier Inc.
Citation
EXPERIMENTAL CELL RESEARCH, v. 280, issue. 2, page. 280-287
Abstract
To identify genes involved in cervical carcinogenesis, the mRNA differential display method was used. A 220-bp cDNA fragment called CA11 was present in normal cervical tissue but not in primary cervical cancer tissue or cervical cancer cell lines. CA11 exhibited 98% homology with the recorded human secreted frizzled-related protein (hsFRP) sequence. A dominant hsFRP mRNA transcript of approximately 4.6 kb was present in three normal cervical tissues examined. Expression of the transcript was nearly absent from three cervical cancer tissues and from five human cervical cancer-derived cell lines. Results from in situ hybridization showed that the hsFRP transcript was confined to the normal cervical epithelial layer. When hsFRP-transfected HeLa and CUMC-6 cervical cancer cells were cultured in serum-free medium, most of the cells died within 8 days. This effect is associated with the apoptotic process. The caspase-3 inhibitor 1, Ac-DEVD-CHO, blocked hsFRP-induced apoptotic cell death. Additionally, cleavage of poly (ADP-ribose) polymerase in hsFRP-transfected cells was confirmed by colorimetric assay. These results indicate that the hsFRP gene probably functions as a tumor suppressor in normal cervical epithelium and down-regulation of hsFRP contributes to development of cervical cancer.
URI
https://www.sciencedirect.com/science/article/pii/S0014482702956491https://repository.hanyang.ac.kr/handle/20.500.11754/157981
ISSN
0014-4827
DOI
10.1006/excr.2002.5649
Appears in Collections:
COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > ETC
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