Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이민형 | - |
dc.date.accessioned | 2021-02-08T02:36:05Z | - |
dc.date.available | 2021-02-08T02:36:05Z | - |
dc.date.issued | 2020-01 | - |
dc.identifier.citation | JOURNAL OF CONTROLLED RELEASE, v. 317, page. 273-281 | en_US |
dc.identifier.issn | 0168-3659 | - |
dc.identifier.issn | 1873-4995 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0168365919306492?via%3Dihub | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/157916 | - |
dc.description.abstract | Antisense miRNA oligonucleotides against miR-21 (AMO-21) have a therapeutic potential for treatment of glioblastoma. However, glioblastoma-targeted delivery through systemic injection requires development of an efficient targeting carrier. For this purpose, a glioblastoma-targeting carrier was developed using the T7 peptide and exosomes. The transferrin receptor is overexpressed on the surface of glioblastoma cells, and T7 is a transferrin receptor-binding peptide. A T7 peptide-decorated exosome (T7-exo) was produced by incorporation of T7 into the exosome membrane as a fusion protein of T7 and Lamp2b. As a control, rabies virus glycoprotein (RVG) peptide targeting brain neuron cells was incorporated into the exosome membrane. AMO-21 was loaded into the exosomes by electroporation. In vitro studies of AMO-21 delivery showed that T7-exo had a higher delivery efficiency to C6 glioblastoma cells than unmodified exosome (Unmod-exo) and RVG-decorated exosome (RVG-exo). For in vivo delivery studies, T7-exo with AMO-21 was delivered into intracranial glioblastoma rat models by intravenous injection through the tail vein. The results showed that T7-exo delivered AMO-21 into the brain more efficiently than Unmod-exo and RVG-exo. In addition, delivery of AMO-21 using T7-exo reduced the miR-21 level in the glioblastoma efficiently. Reduction of miR-21 by AMO-21 induced the expression of PDCD4 and PTEN in tumors, resulting in reduction of tumor sizes. Taken together, these findings indicate that T7-exo is an efficient carrier of AMO-21 into the glioblastoma and may be useful in development of glioblastoma therapy. | en_US |
dc.description.sponsorship | This work was supported by the Individual Basic Science & Engineering Research Program (NRF-2017R1A2B4009036) through the National Research Foundation funded by the Ministry of Science and ICT. | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER SCIENCE BV | en_US |
dc.subject | T7 peptide | en_US |
dc.subject | Transferrin receptor | en_US |
dc.subject | Glioblastoma | en_US |
dc.subject | microRNA-21 | en_US |
dc.subject | Anti-microRNA oligonucleotide | en_US |
dc.title | Systemic delivery of microRNA-21 antisense oligonucleotides to the brain using T7-peptide decorated exosomes | en_US |
dc.type | Article | en_US |
dc.relation.volume | 317 | - |
dc.identifier.doi | 10.1016/j.jconrel.2019.11.009 | - |
dc.relation.page | 273-281 | - |
dc.relation.journal | JOURNAL OF CONTROLLED RELEASE | - |
dc.contributor.googleauthor | Kim, Gyeungyun | - |
dc.contributor.googleauthor | Kim, Minkyung | - |
dc.contributor.googleauthor | Lee, Youngki | - |
dc.contributor.googleauthor | Byun, Jung Woo | - |
dc.contributor.googleauthor | Hwang, Do Won | - |
dc.contributor.googleauthor | Lee, Minhyung | - |
dc.relation.code | 2019002890 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | minhyung | - |
dc.identifier.orcid | https://orcid.org/0000-0002-7083-9296 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.