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The functional domains of dopamine transporter for cocaine analog, CFT binding

Title
The functional domains of dopamine transporter for cocaine analog, CFT binding
Author
이영식
Keywords
dopamine transporter (DAT); cocaine; cocaine binding; CFT; CFT binding; transmembrane domain
Issue Date
2002-08
Publisher
NATURE PUBLISHING GROUP
Citation
Experimental & Molecular Medicine, v. 34, no. 1, page. 90-94
Abstract
Cocaine analogue, CFT (2β-carbomethoxy-3β-(4-fluorophenyl) tropane) binding to dopamine transporter (DAT) in different species is quite heterogeneous. CFT is scarcely detected in bovine DAT whereas it is conspicuous in humans. To examine the structural basis for this functional discrepancy, we analyzed transporter chimeras of these two DATs. The CFT binding activities are avid in all of the chimeric DATs of which both of the 3rd and the 6-8th transmembrane domain (TM) are composed of human DAT sequences. On the contrary, CFT binding activities were scarcely detected if either or both of two regions are replaced with bovine sequences. These findings indicate that the CFT binding absolutely requires human DAT sequences, at least, in the regions encompassing the 3rd and 6-8th transmembrane domain (TM), and that these regions might contribute to form the 3-dimensional pocket for CFT binding.
URI
https://www.nature.com/articles/emm200213https://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART001100104https://repository.hanyang.ac.kr/handle/20.500.11754/157470
ISSN
2092-6413; 1226-3613
DOI
10.1038/emm.2002.13
Appears in Collections:
COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > MOLECULAR AND LIFE SCIENCE(분자생명과학과) > Articles
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