Separation of astaxanthin from red yeast Phaffia rhodozyma by supercritical carbon dioxide extraction
- Title
- Separation of astaxanthin from red yeast Phaffia rhodozyma by supercritical carbon dioxide extraction
- Author
- 이은규
- Keywords
- Supercritical fluid extraction; Astaxanthin; Yeastn; Separation; Purification; Cell; disruption
- Issue Date
- 2003-06
- Publisher
- Elsevier B.V.
- Citation
- BIOCHEMICAL ENGINEERING JOURNAL, v. 11, issue. 2-3, page. 181-187
- Abstract
- The supercritical fluid extraction (SFE) behavior was investigated to extract astaxanthin from the red yeast Phaffia rhodozyma, which
was disrupted and dried by bead mill and spray dryer, respectively. The effects of extraction pressure (102.500 bar), temperature (40, 60
and 80 .C), CO2 flow rate (superficial velocities of 0.27 and 0.54 cm/min) and the use of ethyl alcohol as a modifier (1, 5, 10, 15 vol.%)
on the extraction efficiency were determined. The highest yield of carotenoids and astaxanthin with equal amounts of CO2 (50 g) was 84
and 90%, respectively, at 40 .C and 500 bar. Using a two-step pressure gradient operation, on changing the pressure from 300 to 500 bar,
the concentration of astaxanthin in the second fraction at 500 bar increased by about 4 and 10 times at 40 and 60 .C, respectively, and
the concentration of carotenoids increased by about 3.6 times at 40 .C and 13 times at 60 .C with the yield decreasing by about 40.50%.
ⓒ 2002 Elsevier Science B.V. All rights reserved.
- URI
- https://www.sciencedirect.com/science/article/pii/S1369703X02000232https://repository.hanyang.ac.kr/handle/20.500.11754/155913
- ISSN
- 1873-295X
- DOI
- 10.1016/S1369-703X(02)00023-2
- Appears in Collections:
- COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > BIONANO ENGINEERING(생명나노공학과) > Articles
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