Expression and characterization of a novel enantioselective lipase from Acinetobacter species SY-01.Expression and characterization of a novel enantioselective lipase from Acinetobacter species SY-01.Expression and characterization of a novel enantioselective lipase from Acinetobacter species SY-01
- Title
- Expression and characterization of a novel enantioselective lipase from Acinetobacter species SY-01.Expression and characterization of a novel enantioselective lipase from Acinetobacter species SY-01.Expression and characterization of a novel enantioselective lipase from Acinetobacter species SY-01
- Author
- 정일엽
- Keywords
- Acinetobacter speciesSY-01; Lipase; Lipase-specific chaperone; Enantioselective; cis-(±)-2-(bromomethyl)-2-(2,4-dichlorophenyl)-1,3-dioxolane-4-methyl acetat
- Issue Date
- 2003-05
- Publisher
- EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
- Citation
- BIOCHIMIE, v. 85, issue. 5, page. 501-510
- Abstract
- A novel lipase gene, lipase A, of Acinetobacter species SY-01 (A. species SY-01) was cloned, sequenced, and expressed in Bacillus subtilis 168. The deduced amino acid (aa) sequences for the lipase A and its chaperone, lipase-specific chaperone, were found to encode mature proteins of 339 aa (37.2 kDa) and 347 aa (38.1 kDa), respectively. The aa sequence of lipase A and lipase-specific chaperone shared high homology 82 and 67% identity with the lipase A and the lipase B of A. species RAG-1. This new lipase was defined as a group I Proteobacterial lipase family. The expressed lipase A was purified through sequential treatment with Q-Sepharose, Resource Q, and Superdex-S75 columns. The maximal activity was observed at 50 degrees C for hydrolysis of p-nitrophenyl monoesters and found to be stable at pH 9-11, with optimal activity at pH 10. Lipase A hydrolyzed wide range of fatty acid esters of p-nitrophenyl, but preferentially hydrolyzed short length acyl chains (C2 and C4). Moreover, lipase A from A. species SY-01 catalyzed hydrolysis of the two acetate isomers of cis-(+/-)-2-(bromomethyl)-2-(2,4-dichloro phenyl)-1,3-dioxolane-4-methyl acetate, an intermediate required for the synthesis of Itraconazole which was an anti-fungal drug, at different rate and yielded cis-(-)-isomer in 81.5% conversion with 91.9% enantiomeric excess.
- URI
- https://www.sciencedirect.com/science/article/pii/S0300908403000579https://repository.hanyang.ac.kr/handle/20.500.11754/155791
- ISSN
- 0300-9084
- DOI
- 10.1016/S0300-9084(03)00057-9
- Appears in Collections:
- COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > MOLECULAR AND LIFE SCIENCE(분자생명과학과) > Articles
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