Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김정목 | - |
dc.date.accessioned | 2020-11-12T08:06:07Z | - |
dc.date.available | 2020-11-12T08:06:07Z | - |
dc.date.issued | 2019-11 | - |
dc.identifier.citation | INFECTION AND IMMUNITY, v. 87, no. 11, article no. e00312-19 | en_US |
dc.identifier.issn | 0019-9567 | - |
dc.identifier.issn | 1098-5522 | - |
dc.identifier.uri | https://iai.asm.org/content/87/11/e00312-19 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/155484 | - |
dc.description.abstract | The Bacteroides fragilis enterotoxin (BFT), a virulence factor of enterotoxigenic B. fragilis (ETBF), interacts with intestinal epithelial cells and can provoke signals that induce mucosal inflammation. Although beta-catenin signaling is reported to be associated with inflammatory responses and BFT is known to cleave E-cadherin linked with beta-catenin, little is known about the beta-catenin-mediated regulation of inflammation in ETBF infection. This study was conducted to investigate the role of beta-catenin as a cellular signaling intermediate in the induction of proinflammatory responses to stimulation of intestinal epithelial cells with BFT. Expression of beta-catenin in intestinal epithelial cells was reduced relatively early after stimulation with BFT and then recovered to normal levels relatively late after stimulation. In contrast, phosphorylation of beta-catenin in BFT-exposed cells occurred at high levels early in stimulation and decreased as time passed. Concurrently, late after stimulation the nuclear levels of beta-catenin were relatively higher than those early after stimulation. Suppression of beta-catenin resulted in increased NF-kappa B activity and interleukin-8 (IL-8) expression in BFT-stimulated cells. However, suppression or enhancement of beta-catenin expression neither altered the phosphorylated I kappa B kinase alpha/beta complex nor activated activator protein 1 signals. Furthermore, inhibition of glycogen synthase kinase 3 beta was associated with increased beta-catenin expression and attenuated NF-kappa B activity and IL-8 expression in BFT-exposed cells. These findings suggest the negative regulation of NF-kappa B-mediated inflammatory responses by beta-catenin in intestinal epithelial cells stimulated with BFT, resulting in attenuation of acute inflammation in ETBF infection. | en_US |
dc.description.sponsorship | This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education, Science and Technology (MEST) (grant NRF-2018R1D1A1B07043350), Republic of Korea. | en_US |
dc.language.iso | en | en_US |
dc.publisher | AMER SOC MICROBIOLOGY | en_US |
dc.subject | Bacteroides fragilis | en_US |
dc.subject | enterotoxin | en_US |
dc.subject | intestinal epithelial cells | en_US |
dc.subject | NK-kappa B | en_US |
dc.subject | beta-catenin | en_US |
dc.title | Intestinal Epithelial Cells Exposed to Bacteroides fragilis Enterotoxin Regulate NF-kappa B Activation and Inflammatory Responses through beta-Catenin Expression | en_US |
dc.type | Article | en_US |
dc.relation.no | 11 | - |
dc.relation.volume | 87 | - |
dc.identifier.doi | 10.1128/IAI.00312-19 | - |
dc.relation.page | 1-16 | - |
dc.relation.journal | INFECTION AND IMMUNITY | - |
dc.contributor.googleauthor | Jeon, Jong Ik | - |
dc.contributor.googleauthor | Ko, Su Hyuk | - |
dc.contributor.googleauthor | Kim, Jung Mogg | - |
dc.relation.code | 2019001226 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | jungmogg | - |
dc.identifier.researcherID | D-3112-2015 | - |
dc.identifier.orcid | https://orcid.org/0000-0002-6506-7519 | - |
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