86 38

Increased amounts and stability of telomeric repeat-containing RNA (TERRA) following DNA damage induced by etoposide

Title
Increased amounts and stability of telomeric repeat-containing RNA (TERRA) following DNA damage induced by etoposide
Author
호정규
Keywords
TRF2; TRANSCRIPTION; PROTECTION; ATM; P53
Issue Date
2019-11
Publisher
PUBLIC LIBRARY SCIENCE
Citation
PLOS ONE, v. 14, no. 11, article no. e0225302
Abstract
Telomeric repeat-containing RNAs (TERRAs) are long noncoding RNAs transcribed from subtelomeres toward telomeric repeat tracts, which have been implicated in telomere protection and heterochromatin formation. Genotoxic stress leads to upregulation of TERRAs. However, the mechanism of DNA damage-mediated TERRA induction remains elusive. Here, we treated HeLa cells with etoposide, a DNA double-strand break-generating agent, for various times and monitored the levels of TERRAs. Etoposide treatment led to a gradual time-dependent increase in TERRAs. Etoposide-mediated induction was evident in many TERRAs arising from various chromosome loci, including 20q and XpYp. Chromatin immunoprecipitation assays revealed no significant changes in the occupancy of RNA polymerase II at telomeres upon etoposide treatment. Interestingly, TERRAs arising from 20q, XpYp, 10q, and 13q degraded at slower rates in cells treated with etoposide, while degradation rates of TERRAs from many loci tested were nearly identical in both etoposide- and mock-treated cells. Telomere damage occurred from early time points of etoposide treatment, but telomere lengths and abundance of telomeric repeat-binding factor 2 (TRF2) at telomeres remained unchanged. In summary, etoposide treatment led to telomere damage and TERRA accumulation, but telomere lengths and TRF2-mediated telomere integrity were maintained. Etoposide-mediated TERRA accumulation could be attributed partly to RNA stabilization. These findings may provide insight into the post-transcriptional regulation of TERRAs in response to DNA damage.
URI
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0225302https://repository.hanyang.ac.kr/handle/20.500.11754/155018
ISSN
1932-6203
DOI
10.1371/journal.pone.0225302
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
Files in This Item:
Increased amounts and stability of telomeric repeat-containing RNA (TERRA) following DNA damage induced by etoposide.pdfDownload
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE