Comparison of MicroRNA Expression in Tears of Normal Subjects and Sjogren Syndrome Patients
- Comparison of MicroRNA Expression in Tears of Normal Subjects and Sjogren Syndrome Patients
- biomarkers; microRNAs; miRNAs; Sjogren syndrome; tear
- Issue Date
- ASSOC RESEARCH VISION OPHTHALMOLOGY INC
- INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, v. 60, no. 14, Page. 4889-4895
- PURPOSE. Deregulated expression of several microRNAs (miRNAs) in sera or salivary glands of patients with Sjogren syndrome (SS) has been reported. However, none have investigated miRNAs in samples that can represent lacrimal glands. We compared the miRNAs expression in the tears of SS patients and healthy controls. Moreover, we investigated the correlation between miRNAs expression and ocular staining score (OSS).
METHODS. Individual tear samples were collected from 18 SS patients and 8 age-matched controls. Clinical ophthalmologic assessments included Schirmer I test, tear film breakup time (tBUT), and OSS. The expression of 43 different miRNAs in tears was measured using real-time polymerase chain reaction, and compared between the SS patients and controls. And we also compared between the three groups of control, primary SS, and secondary SS patients. The correlation between the miRNA expression and OSS was evaluated.
RESULTS. The expression levels of miR-16-5p, miR-34a-5p, miR-142-3p, and miR-223-3p were significantly upregulated in patients with SS when compared with those in the control group (P ˂ 0.05). The expression of 10 miRNAs (miR-30b-5p, miR-30c-5p, miR-30d-5p, miR-92a-3p, miR-134-5p, miR-137, miR-302d-5p, miR-365b-3p, miR-374c-5p, miR-487b-3p) was significantly downregulated in the SS patients (P ˂ 0.05). Eight miRNAs showed statistically significant differences between the three groups of control, primary SS and secondary SS. All 14 miRNAs with significant differences in SS patients and control group were not significantly correlated with OSSs.
CONCLUSIONS. The 14 differentially expressed miRNAs may be involved in the pathogenesis of SS, in particular, related to autoinamunity and neuropathy.
- 0146-0404; 1552-5783
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