Purification of soluble human epidermal growth factor (hEGF) from recombinant Escherichia coli culture broth by using expanded‐bed adsorption chromatography
- Title
- Purification of soluble human epidermal growth factor (hEGF) from recombinant Escherichia coli culture broth by using expanded‐bed adsorption chromatography
- Author
- 이은규
- Keywords
- expanded‐bed adsorption (EBA); Escherichia coli fermentation; human epidermal growth factor (hEGF); protein purification; scale‐up
- Issue Date
- 2003-02
- Publisher
- PORTLAND PRESS
- Citation
- BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY V.38, No,1, Page.9-13
- Abstract
- Human epidermal growth factor (hEGF) secreted by recombinant Escherichia coli was purified from culture broth by expanded‐bed adsorption (EBA) chromatography, strong anion‐exchange chromatography and finally preparative reversed‐phase HPLC (RP‐HPLC). The EBA chromatography step simultaneously captured the hEGF by cationic exchanger and removed the cellular biomass from the diluted culture broth. This step was carried out at high throughput, and resulted in a high yield (>90%) and a purification factor of approx. 20‐fold to >80% purity. Its process performance was well maintained during a 16‐fold scale‐up. After the successive purification steps of anion‐exchange chromatography and RP‐HPLC, the overall yield was approx. 84% and the purity was satisfactory (>99.5%). It was concluded that the purification process was very efficient and scaleable, warranting its implementation in large‐scale manufacturing.
- URI
- https://repository.hanyang.ac.kr/handle/20.500.11754/154965
- DOI
- 10.1042/BA20020113
- Appears in Collections:
- COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > BIONANO ENGINEERING(생명나노공학과) > Articles
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