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Maintenance of hPSCs under Xeno-Free and Chemically Defined Culture Conditions

Title
Maintenance of hPSCs under Xeno-Free and Chemically Defined Culture Conditions
Author
김계성
Keywords
Embryonic stem cells; Induced pluripotent stem cells; Feeder-free; Chemically defined conditions; Extracellular matrices
Issue Date
2019-11
Publisher
KOREAN SOC STEM CELL RESEARCH
Citation
INTERNATIONAL JOURNAL OF STEM CELLS, v. 12, no. 3, Page. 484-496
Abstract
Previously, the majority of human embryonic stem cells and human induced pluripotent stem cells have been derived on feeder layers and chemically undefined medium. Those media components related to feeder cells, or animal products, often greatly affect the consistency of the cell culture. There are clear advantages of a defined, xeno-free, and feeder-free culture system for human pluripotent stem cells (hPSCs) cultures, since consistency in the formulations prevents lot-to-lot variability. Eliminating all non-human components reduces health risks for downstream applications, and those environments reduce potential immunological reactions from stem cells. Therefore, development of feeder-free hPSCs culture systems has been an important focus of hPSCs research. Recently, researchers have established a variety of culture systems in a defined combination, xeno-free matrix and medium that supports the growth and differentiation of hPSCs. Here we described detailed hPSCs culture methods under feeder-free and chemically defined conditions using vitronetin and TeSR-E8 medium including supplement bioactive lysophospholipid for promoting hPSCs proliferation and maintaining stemness.
URI
http://www.ijstemcell.com/journal/view.html?doi=10.15283/ijsc19090https://repository.hanyang.ac.kr/handle/20.500.11754/154764
ISSN
2005-3606; 2005-5447
DOI
10.15283/ijsc19090
Appears in Collections:
GRADUATE SCHOOL OF BIOMEDICAL SCIENCE AND ENGINEERING[S](의생명공학전문대학원) > TRANSLATIONAL MEDICAL SCIENCE(임상의과학과) > Articles
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