pH-sensitive colorimetric polydiacetylene vesicles for urease sensing
- Title
- pH-sensitive colorimetric polydiacetylene vesicles for urease sensing
- Author
- 김종만
- Keywords
- Urease sensor; Polydiacetylene vesicles; pH-responsive sensor
- Issue Date
- 2019-10
- Publisher
- ELSEVIER SCI LTD
- Citation
- DYES AND PIGMENTS, v. 169, Page. 15-21
- Abstract
- Urease is a virulence-factor enzyme found in various pathogenic bacteria. Many urinary-tract and gastro-intestinal diseases, including cancer, are related to urease activity, indicating that urease is critical to the human body. Herein, we report a colorimetric urease-sensing method using polydiacetylene vesicles. Due to the ammonia formed during urea hydrolyzation, the increase in local pH causes a color transition of the polydiacetylene vesicles from blue to red. Urease detection using this method is rapid, simple, sensitive, and economical. A minimum of 70 pU/ml of urease can be detected after 15 min of incubation, with a linearity range of 20 pU/ml-0.2 mU/ml. Urease detection in simulated gastric fluid was satisfactory, with a recovery percentage of 93.7-109.1%. The method was also used to screen the urease inhibitor acetohydroxamic acid, and the half-maximal inhibition value (IC50) was found to be 50 mM.
- URI
- https://www.sciencedirect.com/science/article/pii/S0143720819305248?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/154652
- ISSN
- 0143-7208; 1873-3743
- DOI
- 10.1016/j.dyepig.2019.04.072
- Appears in Collections:
- COLLEGE OF ENGINEERING[S](공과대학) > CHEMICAL ENGINEERING(화학공학과) > Articles
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