127 67

UPF1/SMG7-dependent microRNA-mediated gene regulation

Title
UPF1/SMG7-dependent microRNA-mediated gene regulation
Author
남진우
Keywords
MESSENGER-RNA DECAY; 3' UNTRANSLATED REGIONS; ALTERNATIVE CLEAVAGE; GLOBAL ANALYSES; UPF1 BINDING; NMD; POLYADENYLATION; SMG5-SMG7; 3'-UTR; DICER
Issue Date
2019-09
Publisher
NATURE PUBLISHING GROUP
Citation
NATURE COMMUNICATIONS, v. 10, article no. 4181
Abstract
The stability and quality of metazoan mRNAs are under microRNA (miRNA)-mediated and nonsense-mediated control. Although UPF1, a core mediator of nonsense-mediated mRNA decay (NMD), mediates the decay of target mRNA in a 3'UTR-length-dependent manner, the detailed mechanism remains unclear. Here, we suggest that 3'UTR-length-dependent mRNA decay is not mediated by nonsense mRNAs but rather by miRNAs that downregulate target mRNAs via Ago-associated UPF1/SMG7. Global analyses of mRNAs in response to UPF1 RNA interference in miRNA-deficient cells reveal that 3'UTR-length-dependent mRNA decay by UPF1 requires canonical miRNA targeting. The destabilization of miRNA targets is accomplished by the combination of Agog and UPF1/SMG7, which may recruit the CCR4-NOT deadenylase complex. Indeed, loss of the SMG7-deadenylase complex interaction increases the levels of transcripts regulated by UPF1-SMG7. This UPF1/SMG7-dependent miRNA-mediated mRNA decay pathway may enable miRNA targeting to become more predictable and expand the miRNA-mRNA regulatory network.
URI
https://www.nature.com/articles/s41467-019-12123-7https://repository.hanyang.ac.kr/handle/20.500.11754/153949
ISSN
2041-1723
DOI
10.1038/s41467-019-12123-7
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
Files in This Item:
UPF1 SMG7-dependent microRNA-mediated gene regulation.pdfDownload
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE