Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 황정욱 | - |
dc.date.accessioned | 2020-09-16T00:43:19Z | - |
dc.date.available | 2020-09-16T00:43:19Z | - |
dc.date.issued | 2019-09 | - |
dc.identifier.citation | NATURE COMMUNICATIONS, v. 10, article no. 4181 | en_US |
dc.identifier.issn | 2041-1723 | - |
dc.identifier.uri | https://www.nature.com/articles/s41467-019-12123-7 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/153948 | - |
dc.description.abstract | The stability and quality of metazoan mRNAs are under microRNA (miRNA)-mediated and nonsense-mediated control. Although UPF1, a core mediator of nonsense-mediated mRNA decay (NMD), mediates the decay of target mRNA in a 3'UTR-length-dependent manner, the detailed mechanism remains unclear. Here, we suggest that 3'UTR-length-dependent mRNA decay is not mediated by nonsense mRNAs but rather by miRNAs that downregulate target mRNAs via Ago-associated UPF1/SMG7. Global analyses of mRNAs in response to UPF1 RNA interference in miRNA-deficient cells reveal that 3'UTR-length-dependent mRNA decay by UPF1 requires canonical miRNA targeting. The destabilization of miRNA targets is accomplished by the combination of Agog and UPF1/SMG7, which may recruit the CCR4-NOT deadenylase complex. Indeed, loss of the SMG7-deadenylase complex interaction increases the levels of transcripts regulated by UPF1-SMG7. This UPF1/SMG7-dependent miRNA-mediated mRNA decay pathway may enable miRNA targeting to become more predictable and expand the miRNA-mRNA regulatory network. | en_US |
dc.description.sponsorship | We thank Dr. Narry Kim (Seoul National University) for the pCK-FLAG-Ago2 plasmid. This work was supported by National Research Foundation of Korea (NRF) grants funded by the Korean Government (2017M3A9C8028794 to J.H., 2017M3A9G8084539 to J.N., 2018R1A2B2003782 to J.N.) and by a grant from the Medical Research Center (2017R1A5A2015395 to J.H.) funded by the NRF of Korea of the Ministry of Science and ICT, Republic of Korea. | en_US |
dc.language.iso | en | en_US |
dc.publisher | NATURE PUBLISHING GROUP | en_US |
dc.subject | MESSENGER-RNA DECAY | en_US |
dc.subject | 3' UNTRANSLATED REGIONS | en_US |
dc.subject | ALTERNATIVE CLEAVAGE | en_US |
dc.subject | GLOBAL ANALYSES | en_US |
dc.subject | UPF1 BINDING | en_US |
dc.subject | NMD | en_US |
dc.subject | POLYADENYLATION | en_US |
dc.subject | SMG5-SMG7 | en_US |
dc.subject | 3'-UTR | en_US |
dc.subject | DICER | en_US |
dc.title | UPF1/SMG7-dependent microRNA-mediated gene regulation | en_US |
dc.type | Article | en_US |
dc.relation.no | 1 | - |
dc.relation.volume | 10 | - |
dc.identifier.doi | 10.1038/s41467-019-12123-7 | - |
dc.relation.page | 1-15 | - |
dc.relation.journal | NATURE COMMUNICATIONS | - |
dc.contributor.googleauthor | Park, Jungyun | - |
dc.contributor.googleauthor | Seo, Jwa-Won | - |
dc.contributor.googleauthor | Ahn, Narae | - |
dc.contributor.googleauthor | Park, Seokju | - |
dc.contributor.googleauthor | Hwang, Jungwook | - |
dc.contributor.googleauthor | Nam, Jin-Wu | - |
dc.relation.code | 2019001121 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL OF BIOMEDICAL SCIENCE AND ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOMEDICAL SCIENCE | - |
dc.identifier.pid | jwhwang | - |
dc.identifier.researcherID | P-1614-2015 | - |
dc.identifier.orcid | https://orcid.org/0000-0002-2290-1649 | - |
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