Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김용희 | - |
dc.date.accessioned | 2020-08-25T08:00:49Z | - |
dc.date.available | 2020-08-25T08:00:49Z | - |
dc.date.issued | 2019-09 | - |
dc.identifier.citation | GENOME RESEARCH, v. 29, no. 9, Page. 1442-1452 | en_US |
dc.identifier.issn | 1088-9051 | - |
dc.identifier.issn | 1549-5469 | - |
dc.identifier.uri | https://genome.cshlp.org/content/29/9/1442 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/152534 | - |
dc.description.abstract | Obesity is an increasing pathophysiological problem in developed societies. Despite all major progress in understanding molecular mechanisms of obesity, currently available anti-obesity drugs have shown limited efficacy with severe side effects. CRISPR interference (CRISPRi) mechanism based on catalytically dead Cas9 (dCas9) and single guide RNA (sgRNA) was combined with a targeted nonviral gene delivery system to treat obesity and obesity-induced type 2 diabetes. A fusion peptide targeting a vascular and cellular marker of adipose tissue, prohibitin, was developed by conjugation of adipocyte targeting sequence (CKGGRAKDC) to 9-mer arginine (ATS-9R). (dCas9/sgFabp4) + ATS-9R oligoplexes showed effective condensation and selective delivery into mature adipocytes. Targeted delivery of the CRISPRi system against Fabp4 to white adipocytes by ATS-9R induced effective silencing of Fabp4, resulting in reduction of body weight and inflammation and restoration of hepatic steatosis in obese mice. This RNA-guided DNA recognition platform provides a simple and safe approach to regress and treat obesity and obesity-induced metabolic syndromes. | en_US |
dc.description.sponsorship | This research was partially supported by grants from the National Research Foundation of Korea (NRF-2015R1A2A1A09003019, NRF-2019R1A2C3008992) and Bio & Medical technology development program (NRF-2017M3A9F5029655), the research fund of Hanyang University (HY-201800000000282), the Brain Korea 21 plus program (22A20130011095), and the Korean Health Technology R&D project through the Ministry of Health and Welfare (HI17C0888). | en_US |
dc.language.iso | en | en_US |
dc.publisher | COLD SPRING HARBOR LAB PRESS | en_US |
dc.subject | SEQUENCE-SPECIFIC CONTROL | en_US |
dc.subject | ADIPOSE-TISSUE | en_US |
dc.subject | HUMAN-CELLS | en_US |
dc.subject | ADIPOKINES | en_US |
dc.title | Targeted delivery of CRISPR interference system against Fabp4 to white adipocytes ameliorates obesity, inflammation, hepatic steatosis, and insulin resistance | en_US |
dc.type | Article | en_US |
dc.relation.no | 9 | - |
dc.relation.volume | 29 | - |
dc.identifier.doi | 10.1101/gr.246900.118 | - |
dc.relation.page | 1442-1452 | - |
dc.relation.journal | GENOME RESEARCH | - |
dc.contributor.googleauthor | Chung, Jee Young | - |
dc.contributor.googleauthor | Ul Ain, Qurrat | - |
dc.contributor.googleauthor | Song, Yoonsung | - |
dc.contributor.googleauthor | Yong, Seok-Beom | - |
dc.contributor.googleauthor | Kim, Yong-Hee | - |
dc.relation.code | 2019003284 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | yongheekim | - |
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