Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 안유민 | - |
dc.date.accessioned | 2020-07-24T06:35:32Z | - |
dc.date.available | 2020-07-24T06:35:32Z | - |
dc.date.issued | 2004-09 | - |
dc.identifier.citation | 대한기계학회논문집A, v.28, No. 9, Page. 1429-1434 | en_US |
dc.identifier.issn | 1226-4873 | - |
dc.identifier.uri | https://information.hanyang.ac.kr/#/eds/detail?an=edskci.ARTI.1068045&dbId=edskci | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/151888 | - |
dc.description.abstract | Immunoassay is one of the important analytical methods for clinical diagnoses and biochemical studies, but needs a long time, troublesome procedures and expensive reagents. In this study, therefore, we propose the micro filter chip with microbe ads for immunoassay, which has pillar structures. The advantage of the proposed micro filter chip is to use simple fabrication process and cheap materials. The mold was made by the photolithography technique with Si wafer and negative photoresist SU-8. The replica was made of PDMS, bonded on the pyrex glass. The micro filter chip consists of inlet channel, filter chamber and outlet channel. HBV (Hepatitius B virus) monoclonal antibody (AgI) labeled with biotin were immobilized onto streptavidin coated beads of 30-50㎛ size. Fluorescein isothiocyanate (FITC)-labeled HBV monoclonal antibody (Ag8) was used to detect HBsAg (Hebatitis B virus surface Antigen), and fluorescence intensity was monitored by epi-fluorescence microscope. In this study, the immune response of less than 30 min was obtained with the use of 100 ㎖ of sample. | en_US |
dc.language.iso | ko_KR | en_US |
dc.publisher | 대한기계학회 | en_US |
dc.subject | Biochip | en_US |
dc.subject | 바이오칩 | en_US |
dc.subject | Immunoassay | en_US |
dc.subject | 면역분석법 | en_US |
dc.subject | Replica Molding | en_US |
dc.subject | 복사몰딩 | en_US |
dc.subject | Microbead | en_US |
dc.subject | 마이크로 비드 | en_US |
dc.subject | Microfilter | en_US |
dc.subject | 마이크로 필터 | en_US |
dc.title | 비드를 이용한 면역분석용 마이크로필터 칩의 제작 | en_US |
dc.title.alternative | Microfilter Chip Fabrication for Bead-Based Immunoassay | en_US |
dc.type | Article | en_US |
dc.relation.journal | 대한기계학회논문집 A | - |
dc.contributor.googleauthor | 이승우 | - |
dc.contributor.googleauthor | 채영규 | - |
dc.contributor.googleauthor | 안유민 | - |
dc.relation.code | 2012100288 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF ENGINEERING SCIENCES[E] | - |
dc.sector.department | DEPARTMENT OF MECHANICAL ENGINEERING | - |
dc.identifier.pid | ahnym | - |
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