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비드를 이용한 면역분석용 마이크로필터 칩의 제작

Title
비드를 이용한 면역분석용 마이크로필터 칩의 제작
Other Titles
Microfilter Chip Fabrication for Bead-Based Immunoassay
Author
안유민
Keywords
Biochip; 바이오칩; Immunoassay; 면역분석법; Replica Molding; 복사몰딩; Microbead; 마이크로 비드; Microfilter; 마이크로 필터
Issue Date
2004-09
Publisher
대한기계학회
Citation
대한기계학회논문집A, v.28, No. 9, Page. 1429-1434
Abstract
Immunoassay is one of the important analytical methods for clinical diagnoses and biochemical studies, but needs a long time, troublesome procedures and expensive reagents. In this study, therefore, we propose the micro filter chip with microbe ads for immunoassay, which has pillar structures. The advantage of the proposed micro filter chip is to use simple fabrication process and cheap materials. The mold was made by the photolithography technique with Si wafer and negative photoresist SU-8. The replica was made of PDMS, bonded on the pyrex glass. The micro filter chip consists of inlet channel, filter chamber and outlet channel. HBV (Hepatitius B virus) monoclonal antibody (AgI) labeled with biotin were immobilized onto streptavidin coated beads of 30-50㎛ size. Fluorescein isothiocyanate (FITC)-labeled HBV monoclonal antibody (Ag8) was used to detect HBsAg (Hebatitis B virus surface Antigen), and fluorescence intensity was monitored by epi-fluorescence microscope. In this study, the immune response of less than 30 min was obtained with the use of 100 ㎖ of sample.
URI
https://information.hanyang.ac.kr/#/eds/detail?an=edskci.ARTI.1068045&dbId=edskcihttps://repository.hanyang.ac.kr/handle/20.500.11754/151888
ISSN
1226-4873
Appears in Collections:
COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > MECHANICAL ENGINEERING(기계공학과) > Articles
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