Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이은규 | - |
dc.date.accessioned | 2020-07-21T05:33:29Z | - |
dc.date.available | 2020-07-21T05:33:29Z | - |
dc.date.issued | 2004-09 | - |
dc.identifier.citation | Analytical Sciences, v. 20, No. 9, Page. 1255-1258 | en_US |
dc.identifier.issn | 1348-2246 | - |
dc.identifier.issn | 0910-6340 | - |
dc.identifier.issn | 0910-6340 | - |
dc.identifier.uri | https://information.hanyang.ac.kr/#/eds/detail?an=edselc.2-52.0-5644290746&dbId=edselc | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/151807 | - |
dc.description.abstract | The immobilization of glucose oxidase (GOx), using self assembled monolayers (SAMs) on gold surfaces, was investigated by grazing angle FT-IR spectroscopy, surface plasmon resonance (SPR) spectroscopy, and atomic force microscopy (AFM) in conjunction with confocal laser scanning microscopy (CLSM). To find an optimum condition for the maximum GOx loading density on gold surfaces, different cleaning protocols were examined. The loading density of GOx on surfaces was investigated by AFM and CLSM. In particular, CLSM was more effective for identifying the GOx density than AFM, since its scanning speed is much faster and it covers a larger area. Based on CLSM images of the GOx immobilized on the surfaces, it was concluded that the pre-cleaning process of gold substrates using different solvents, such as acetone, ethanol and 2-propanol, is very important for enhancing the GOx loading density. This result enables us to investigate an effective fabrication process in fabricating biosensors. | en_US |
dc.description.sponsorship | This research was supported by a grant (M102KN01-0001-02K1401-00520) from Center for Nanoscale Mechatronics & Manufacturing, one of 21st Centry Froniter Research Programs, Ministry of Science and Technology, Korea. J.C. thanks the Korean Science and Engineering Foundation (Grant Number R14-2002-004-01000) for financial support. H.L. thanks for the financial support from National R&D Project for Nano Science and Technology (Contract No. M10214000110-02B1500-01800) of MOST. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Japan Society for Analytical Chemistry | en_US |
dc.subject | glucose oxidase | en_US |
dc.subject | gold | en_US |
dc.subject | analytic method | en_US |
dc.subject | article | en_US |
dc.subject | biosensor | en_US |
dc.subject | confocal laser microscopy | en_US |
dc.subject | contrast enhancement | en_US |
dc.subject | density | en_US |
dc.subject | loading test | en_US |
dc.subject | process design | en_US |
dc.subject | protein analysis | en_US |
dc.subject | protein protein interaction | en_US |
dc.subject | spectroscopy | en_US |
dc.subject | surface plasmon resonance | en_US |
dc.title | Enhancement of the Protein Loading Density by a Pre-Cleaning Process of a Gold Substrate: Confocal Laser Scanning Microscopic Study | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.2116/analsci.20.1255 | - |
dc.relation.journal | ANALYTICAL SCIENCES | - |
dc.contributor.googleauthor | PARK, Taehan | - |
dc.contributor.googleauthor | CHOO, Jaebum | - |
dc.contributor.googleauthor | LEE, Moonkwon | - |
dc.contributor.googleauthor | KIM, Yang S. | - |
dc.contributor.googleauthor | LEE, Hae Seong | - |
dc.contributor.googleauthor | Lee, E.K. | - |
dc.relation.code | 2007200601 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF ENGINEERING SCIENCES[E] | - |
dc.sector.department | DEPARTMENT OF BIONANO ENGINEERING | - |
dc.identifier.pid | eklee | - |
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