Enhancement of the Protein Loading Density by a Pre-Cleaning Process of a Gold Substrate: Confocal Laser Scanning Microscopic Study
- Title
- Enhancement of the Protein Loading Density by a Pre-Cleaning Process of a Gold Substrate: Confocal Laser Scanning Microscopic Study
- Author
- 이은규
- Keywords
- glucose oxidase; gold; analytic method; article; biosensor; confocal laser microscopy; contrast enhancement; density; loading test; process design; protein analysis; protein protein interaction; spectroscopy; surface plasmon resonance
- Issue Date
- 2004-09
- Publisher
- Japan Society for Analytical Chemistry
- Citation
- Analytical Sciences, v. 20, No. 9, Page. 1255-1258
- Abstract
- The immobilization of glucose oxidase (GOx), using self assembled monolayers (SAMs) on gold surfaces, was investigated by grazing angle FT-IR spectroscopy, surface plasmon resonance (SPR) spectroscopy, and atomic force microscopy (AFM) in conjunction with confocal laser scanning microscopy (CLSM). To find an optimum condition for the maximum GOx loading density on gold surfaces, different cleaning protocols were examined. The loading density of GOx on surfaces was investigated by AFM and CLSM. In particular, CLSM was more effective for identifying the GOx density than AFM, since its scanning speed is much faster and it covers a larger area. Based on CLSM images of the GOx immobilized on the surfaces, it was concluded that the pre-cleaning process of gold substrates using different solvents, such as acetone, ethanol and 2-propanol, is very important for enhancing the GOx loading density. This result enables us to investigate an effective fabrication process in fabricating biosensors.
- URI
- https://information.hanyang.ac.kr/#/eds/detail?an=edselc.2-52.0-5644290746&dbId=edselchttps://repository.hanyang.ac.kr/handle/20.500.11754/151807
- ISSN
- 1348-2246; 0910-6340; 0910-6340
- DOI
- 10.2116/analsci.20.1255
- Appears in Collections:
- COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > BIONANO ENGINEERING(생명나노공학과) > Articles
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