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HPLC method for simultaneous determination of cefprozil diastereomers in human plasma

Title
HPLC method for simultaneous determination of cefprozil diastereomers in human plasma
Author
김진기
Keywords
Cefprozil; Stereoisomer; Cis; Trans
Issue Date
2004-09
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Citation
Journal of Pharmaceutical and Biomedical Analysis, v. 36, No. 1, Page. 243-248
Abstract
A high-performance liquid chromatography method was developed for the determination of cefprozil diastereomers in human plasma. Cefprozil exists as cis and trans isomer at the ratio of 90:10. Plasma samples were prepared by protein precipitation using acetonitrile, trichloroacetic acid and methylene chloride. After the mixtures were vortexed and centrifuged, the aqueous supernatant was injected into a reversed-phase C8 column. The mobile phase consisted of acetonitrile, glacial acetic acid and distilled water at the volume ratio of 5.5:1.75:92.75 (pH 2.7). The signals were monitored with UV detection at 280 nm. The calibration curves of cis and trans isomer were linear in concentration ranges of 0.1–25 and 0.02–2.5 μg/mL with the correlation coefficient of 0.9999 and 0.9989, respectively. After oral administration of cefprozil in humans, Cmax and Tmax of total cefprozil were 18.80 ± 2.14 μg/mL and 2.06 ± 0.62 h. This method was sensitive with excellent selectivity and reproducibility, and successfully applied to a bioavailability study of cefprozil in healthy subjects.
URI
https://information.hanyang.ac.kr/#/eds/detail?an=edsovi.00080647.200409210.00033&dbId=edsovihttps://repository.hanyang.ac.kr/handle/20.500.11754/151751
ISSN
0731-7085
DOI
10.1016/j.jpba.2004.06.001
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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