Exonuclease requirements for mammalian ribosomal RNA biogenesis and surveillance
- Title
- Exonuclease requirements for mammalian ribosomal RNA biogenesis and surveillance
- Author
- 최준호
- Keywords
- PERLMAN SYNDROME; EXORIBONUCLEASE DIS3L2; PRERIBOSOMAL RNA; QUALITY-CONTROL; NONCODING RNAS; CLEAVAGE SITES; YEAST EXOSOME; STEM-LOOP; DEGRADATION; PATHWAY
- Issue Date
- 2019-06
- Publisher
- NATURE PUBLISHING GROUP
- Citation
- NATURE STRUCTURAL & MOLECULAR BIOLOGY, v. 26, no. 6, Page. 490-490
- Abstract
- Ribosomal RNA (rRNA) biogenesis is a multistep process requiring several nuclear and cytoplasmic exonucleases. The exact processing steps for mammalian 5.8S rRNA remain obscure. Here, using loss-of-function approaches in mouse embryonic stem cells (mESCs) and deep sequencing of rRNA intermediates, we investigate the requirements of exonucleases known to be involved in 5.8S maturation at nucleotide resolution and explore the role of the Perlman syndrome-associated 3'-5' exonuclease Dis3l2 in rRNA processing. We uncover a novel cytoplasmic intermediate that we name '7S(B)' rRNA that is generated through sequential processing by distinct exosome complexes. 7S(B) rRNA can be oligoadenylated by an unknown enzyme and/or oligouridylated by TUT4/7 and subsequently processed by Dis3l2 and Eri1. Moreover, exosome depletion triggers Dis3l2-mediated decay (DMD) as a surveillance pathway for rRNAs. Our data identify previously unknown 5.8S rRNA processing steps and provide nucleotide-level insight into the exonuclease requirements for mammalian rRNA processing.
- URI
- https://www.nature.com/articles/s41594-019-0234-xhttps://repository.hanyang.ac.kr/handle/20.500.11754/151428
- ISSN
- 1545-9993; 1545-9985
- DOI
- 10.1038/s41594-019-0234-x
- Appears in Collections:
- COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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