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dc.contributor.author이영한-
dc.date.accessioned2020-04-22T11:34:24Z-
dc.date.available2020-04-22T11:34:24Z-
dc.date.issued2004-07-
dc.identifier.citationJOURNAL OF MOLECULAR BIOLOGY, v.340, No. 4, Page. 681-693en_US
dc.identifier.issn00222836-
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0022283604004103-
dc.identifier.urihttps://repository.hanyang.ac.kr/handle/20.500.11754/151205-
dc.description.abstractProtein kinase C δ (PKCδ) plays an important role in the regulation of apoptosis in response to diverse anticancer agents. PKCδ is cleaved irreversibly to a catalytically active fragment in response to apoptotic stimuli; however, little information is available about the regulation of PKCδ gene expression. In this study, we found that the amount of steady-state PKCδ mRNA and protein was increased by etoposide in mouse L1210 leukemia cells. The transcriptional rate of the PKCδ gene and the stability of PKCδ mRNA were increased by treatment with etoposide, resulting in the accumulation of PKCδ protein. Rottlerin inhibited etoposide-induced PKCδ gene expression significantly, while Go6976, LY294002 and PD98059 had no effect. Further, both stable and adenovirus-mediated expression of a dominant negative PKCδ(KR) abrogated etoposide-induced PKCδ expression. Etoposide-stimulated PKCδ transcription but not PKCδ mRNA stability was blocked completely by pretreatment with rottlerin. Our data reveal a novel mechanism whereby PKCδ gene is regulated at the transcriptional and post-transcriptional level in the L1210 leukemia cell line.en_US
dc.description.sponsorshipAcknowledgementsWe thank Dr Sung Ho Ryu (Pohang Universityof Science and Technology, Korea) for helpful dis-cussion and comments, and Dr Jang-Soo Chun(Kwangju Institute of Science and Technology,Korea) for providing the plasmids pMTH/PKCdand pMTH/PKCd(KR). This work was supportedby Grant R01-2002-00000167-0 from the BasicResearch Program of the Korea Science andEngineering Foundationen_US
dc.language.isoen_USen_US
dc.publisherACADEMIC PRESS LTDen_US
dc.subjectPKCδen_US
dc.subjectetoposideen_US
dc.subjecttranscriptionen_US
dc.subjectpost-transcriptionen_US
dc.subjectautoregulationen_US
dc.titleTranscriptional and post-transcriptional regulation of PKCdelta gene by etoposide in L1210 murine leukemia cellsen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.jmb.2004.04.006-
dc.relation.journalJOURNAL OF MOLECULAR BIOLOGY-
dc.contributor.googleauthorShin, Soon Young-
dc.contributor.googleauthorKim, Chang Gun-
dc.contributor.googleauthorKo, Jesang-
dc.contributor.googleauthorMin, Do Sik-
dc.contributor.googleauthorChang, Jong-soo-
dc.contributor.googleauthorOhba, Motoi-
dc.contributor.googleauthorKuroki, Toshio-
dc.contributor.googleauthorChoi, Young Bong-
dc.contributor.googleauthorKim, Young-Ho-
dc.contributor.googleauthorLee, Young Han-
dc.relation.code2009205443-
dc.sector.campusE-
dc.sector.daehakCOLLEGE OF SCIENCE & TECHNOLOGY[E]-
dc.sector.departmentDIVISION OF MOLECULAR & LIFE SCIENCE-
dc.identifier.pidyounghan-
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COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > ETC
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