항체와 결합이 가능한 단백질 A를 이용한 약물전달

Abstract

Specific delivery to target cells and efficient cellular uptake of protein, drug and small interfering RNA(siRNA) remains major challenges for gene-targeted therapies. Here we designed and prepared a fusion protein (ZZ-cys, ZZ-GFP and ZZ-HSP27), as an IgG-capturing protein to enhance incorporation of antibody. First, we confirmed the binding ability between the target specific antibody and ZZ proteins. To selectively deliver protein, ZZ fusion protein was bound with specific antibody (Anti-CD7 antibody or Anti-Her2/neu antibody) against target cells (Jurkat and SKBR3). The results showed that Anti-CD7 antibody-ZZ GFP complex or Anti-Her/neu antibody-ZZ GFP complex delivers protein into Jurkat cells or SKBR3 cells, respectively. Second, we tested the protective effect of Anti-CD7 antibody-ZZ HSP27 fusion protein complex on hypoxia condition and apoptosis induced by cobalt chloride using Annexin V staining and CCK assay. Pre-treatment of Antibody-ZZ HSP27 fusion protein complex resulted in a decrease of apoptosis compared to cells treated with cobalt chloride or only ZZ-HSP27. In this study, we demonstrate that ZZ fusion protein bound with specific antibodies, our new multi-targetable drug delivery system, which can allow to selectively change a target cell by simply exchange of targeting antibody. Thus, Our new universal drug delivery system would be a powerful tool for therapeutic protein delivery.