Change of cytokines in aqueous humor in exudative age related macular degeneration

Abstract

Exudative Age related macular degeneration (AMD) with characteristic Choroidal neovascularization (CNV) causes irreversible loss in visual acuity in elderly patients. This clinical case series investigated the levels of various cytokines in the aqueous humors of patients with CNV secondary to AMD and normal control cataract patients. A total of 38 aqueous humor samples from 35 diseased patients and 29 controls were collected. No statistical difference was noted in the mean age of age related macular degeneration group (67.9±8.7years), Control group (64.4±10.7 years). Increase in T-cell cytokine receptor (TCCR), also known as WSX-1 was quantitatively analyzed through immunoblot assay using RayBio?? Biotin Label-based Human Antibody Array I. Angiogenesis was induced by treating recombinant human TCCR/WSX-1/Fc chimera protein on primary human umbilical vein endothelial cells (HUVECs). TCCR/WSX-1 theoretically is thought to inhibit antiangiogenic effect of IL-27 or induce angiogenesis as in a soluble form.