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운동신경세포주에서 재조합 인간 에리스로포이에틴(EPO)에 의한 돌연변이 SOD1/G93A단백질의 침전 억제 효과

Title
운동신경세포주에서 재조합 인간 에리스로포이에틴(EPO)에 의한 돌연변이 SOD1/G93A단백질의 침전 억제 효과
Other Titles
Recombinant human erythropoietin attenuates mutant Cu/Zn-binding superoxide dismutase(SOD1/G93A) aggregation
Author
김가영
Alternative Author(s)
Kim ga young
Advisor(s)
김승현
Issue Date
2012-02
Publisher
한양대학교
Degree
Master
Abstract
Human erythropoietin (hEPO) has multiple actions in non-hematopoietic tissues, including neurotrophic, anti-oxidant, anti-apoptotic, and anti-inflammatory effects. To examine the effect of rhEPO in an vitro model of amyotrophic lateral sclerosis (ALS), we stably overexpressed wild SOD1 and a mutant form, SOD1/G93A, in NSC-34 motoneuron-like cells. Transformants harboring the wild and mutant forms of SOD1 were selected by G418 selection and immunoblot analysis. RT-PCR analysis showed that cox-2 expression was increased in the NSC-34/mSOD1 cells, and MTT assays and BrdU-ELISAs revealed reduced cell growth and proliferation in the NSC-34/mSOD1 cell line. Incubation with 5 or 10 IU/ml rhEPO increased the viability and decreased the cox-2 expression in the dNSC-34/mSOD1 cells. Immunocytochemical staining with anti-SOD1 antibody revealed the presence of aggregates of mSOD1 protein in dNSC-34/mSOD1 cells. Incubation with10 IU/ml rhEPO reduced the proportion of cells containing such aggregates. Our findings suggest that the anti-oxidant and anti-inflammatory effects of rhEPO increase the survival of NSC-34/mSOD1 cells and reduce aggregation of the mutant SOD1 protein.
URI
https://repository.hanyang.ac.kr/handle/20.500.11754/137614http://hanyang.dcollection.net/common/orgView/200000419169
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > DEPARTMENT OF BIOMEDICAL SCIENCES(의생명공학과) > Theses (Master)
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