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Simultaneous determination of cefatrizine and clavulanic acid in dog plasma by HPLC

Title
Simultaneous determination of cefatrizine and clavulanic acid in dog plasma by HPLC
Author
최한곤
Keywords
Cefatrizine; Clavulanic acid; 1,2,4-Triazole; LACTAMASE INHIBITOR COMBINATIONS; BETA-LACTAMASE; CHROMATOGRAPHIC METHODS; BIOANALYTICAL METHODS; VALIDATION; URINE; SUSCEPTIBILITY; INFECTIONS; RESISTANCE; ASSAY
Issue Date
2004-04
Publisher
ELSEVIER SCIENCE BV
Citation
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, v. 35, No. 1, Page. 221-231
Abstract
A rapid and specific high-performance liquid chromatographic method was developed and validated for the simultaneous determination of cefatrizine and clavulanic acid in the plasma of beagle dog. The sample pretreatment procedure involved reaction of clavulanic acid with 1,2,4-triazole, which readily produced a derivative with its maximum UV absorption at 314 nm. This derivative was separated in a reverse-phase C-18 column without being interfered by other components present in plasma. Cefatrizine, however, was not derivatized and, therefore determined directly at 269 nm. Sulfanilamide was used as an internal standard. The retention times of sulfanilamide, the derivative, and cefatrizine were, 3.5, 4.9, and 6.0 min, respectively. The assay showed linearity from 2 to 100 μg/ml for cefatrizine and from 1 to 50 μg/ml for clavulanic acid. Precision expressed as R.S.D. ranged from 4.2 to 18.2% for cefatrizine and 5.5 to 15.8% for clavulanic acid. Accuracy ranged from 97.9 to 120% (lower limit of quantitation) for cefatrizine and from 97.7 to 119.2% for clavulanic acid. Extraction efficiencies for cefatrizine, clavulanic acid, and internal standard from dog plasma averaged 79.8±5.8%, 84.8±6.2%, and 89.0±3.8%, respectively. This method was employed successfully to follow the time course of the concentration of cefatrizine and clavulanic acid in beagle dogs following oral administration of cefatrizine and clavulanic acid.
URI
https://www.sciencedirect.com/science/article/pii/S0731708504000147#!https://repository.hanyang.ac.kr/handle/20.500.11754/136799
ISSN
0731-7085
DOI
10.1016/j.jpba.2004.01.010Get rights and content
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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