Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김진기 | - |
dc.date.accessioned | 2020-03-09T04:43:17Z | - |
dc.date.available | 2020-03-09T04:43:17Z | - |
dc.date.issued | 2004-03 | - |
dc.identifier.citation | JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, v. 27, No. 14, Page. 2293-2306 | en_US |
dc.identifier.issn | 1082-6076 | - |
dc.identifier.issn | 1520-572X | - |
dc.identifier.uri | https://www.tandfonline.com/doi/full/10.1081/JLC-200025726 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/134656 | - |
dc.description.abstract | A sensitive high performance liquid chromatography (HPLC) method has been developed for the determination of dexamethasone in human plasma. After 1.5 mL of plasma was extracted with 4 mL of ethyl acetate containing 80 ng/mL of desoxymethasone, analysis of dexamethasone in plasma samples was carried out using a Sphereclone ODS2 column with UV detection for separation and quantification. A mixture of acetonitrile–10 mM phosphate buffer (pH 7.0) (32:68, v/v) was used as a mobile phase. The limit of quantitative (LOQ) analysis was 10 ng/mL. The accuracy of the assay was from 96.96% to 106.07%, while the intra‐ and inter‐day coefficient of variation of the same concentration range was less than 15%, except LOQ (<20%). The signals were monitored by a UV detector at 240 nm with flow‐rate of 1.0 mL/min. The method could be applied, with great success, to evaluate the bioavailability of dexamethasone in human subjects, with excellent selectivity and reproducibility and clinical study. | en_US |
dc.description.sponsorship | This work was supported by the Korea Food and Drug Administration Grant (KFDA-03142-EQI-501). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | TAYLOR & FRANCIS INC | en_US |
dc.subject | Dexamethasone | en_US |
dc.subject | HPLC | en_US |
dc.subject | Human plasma | en_US |
dc.subject | Bioavailability | en_US |
dc.title | HPLC determination of dexamethasone in human plasma | en_US |
dc.type | Article | en_US |
dc.relation.no | 15 | - |
dc.relation.volume | 27 | - |
dc.identifier.doi | 10.1081/JLC-200025726 | - |
dc.relation.page | 2293-2306 | - |
dc.relation.journal | JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | - |
dc.contributor.googleauthor | Song, Yun‐Kyoung | - |
dc.contributor.googleauthor | Park, Jeong‐Sook | - |
dc.contributor.googleauthor | Kim, Jin‐Ki | - |
dc.contributor.googleauthor | Kim, Chong‐Kook | - |
dc.relation.code | 2007205340 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF PHARMACY[E] | - |
dc.sector.department | DEPARTMENT OF PHARMACY | - |
dc.identifier.pid | jinkikim | - |
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