생화학 분자의 고감도 검출을 위한 나노소재 기반의 전기화학 센싱 플랫폼 개발

Abstract

나노 물질은 지난 10년 사이에 눈부신 발전을 이루며, 나노 물질만이 가지는 고유한 특성을 바탕으로 촉매, 바이오/메디컬 이미징, 센서, 전자 디바이스 등 다양한 영역에서 새로운 지평을 열게하였다. 특히, 1차원 나노 구조체인 탄소나노튜브는 1991년 발견된 이래로 많은 관심과 주목을 받아왔다. 뛰어난 전기 전도성, 우수한 화학 안정성, 높은 표면적-체적 비, 빠른 전자전달 특성 등을 보유한 탄소나노튜브는 바이오 및 환경센서에 이르기까지 전기분석 분야에 광범위하게 이용되고있다. 또한, 탄소나노튜브는 금속 또는 반도체 나노 입자와의 결합을 통해 전기촉매활성 및 전자전달을 향상시켜 빠른 응답속도와 고감도 바이오/케미컬 분자의 검출이 가능한 새로운 전기화학센서 개발의 가능성을 제시하였다. 본 연구에서 우리는 나노입자가 결합된 단일막 탄소나노튜브 전극을 기반으로한 전기화학센싱 응용을 목표로 탄소나노튜브 전극의 미세 패터닝에서부터 나노입자의 증착 및 바이오/케미컬 분자의 전기화학적 검출에 이르기까지 상세히 보고하였다. 1장과 2장에서는 산소 플라즈마와 전기화학 반응을 이용한 두 가지 탄소나노튜브 전극 패터닝 방법에 대해 각각 소개하였고, 3-5장에서는 다양한 나노입자(Au, Pt, ZnO, TiO2)를 탄소나노튜브 전극 상에 증착하는 기술 및 제작된 나노입자/탄소나노튜브 전극을 이용한 바이오/케미컬 분자(glucose, H2O2, hydrazine, arsenic ion)의 전기화학검출 응용에 관해 기술하였다. 특히, 5장에서는 마이크로 플루이딕 시스템과 나노입자/탄소나노튜브 전극을 하나의 칩으로 통합하여 전기화학분석과 마이크로 플루이딕 시스템이 가지는 장점들이 결합된 새롭고 매력적인 센싱 플랫폼으로서의 가능성을 제시하였다.|Nanomaterials have undergone enormous development during the past decade. The unique properties of nanomaterials have opened up new horizons for diverse applications in catalysis, biological imaging, sensors, electronic devices, etc. In particular, carbon nanotubes (CNTs), one-dimensional nanostructures, have attracted much attention since CNTs were discovered in 1991. Owing to their electrical conductance, chemical stability, high surface-to-volume ratio and fast electron transport properties, CNTs are widely used in the electroanalytical fields, including biomedical and environmental sensors. Moreover, the remarkable properties of CNTs in cooperation with metallic or semiconducting nanoparticles (NPs) suggest the possibility of developing novel electrochemical sensing devices for bio/chemical molecules. NPs-modified CNT electrode can allow an enhanced electrocatalytic activity and a promoted electron-transfer reactions, resulting in highly sensitive and fast-response sensing. In this dissertation, we present NPs/single-walled carbon nanotubes (SWCNTs)-based electrochemical sensing application in detail from preparation of patterned SWCNT electrodes to fabrication of NPs/SWCNTs hybrids and electrochemical detection of bio/chemical molecules. In Chapters 1 and 2, two different methods using O2 plasma and electrochemical reaction are introduced for the patterning of SWCNTs films on flexible, transparent plastic substrates. In Chapters 3-5, modification of various nanomaterials (e.g. Au, Pt, ZnO, and TiO2) on SWCNT electrodes is explored for electrochemical applications in bio/chemical molecules sensing (e.g. glucose, hydrogen peroxide, hydrazine and heavy metal). Particularly in Chapter 5, integration of NPs-modified SWCNT electrodes with microfluidic chip is demonstrated as a new attractive sensing platform with significant advantages derived from the combination of electrochemical analysis and microfluidic systems.; Nanomaterials have undergone enormous development during the past decade. The unique properties of nanomaterials have opened up new horizons for diverse applications in catalysis, biological imaging, sensors, electronic devices, etc. In particular, carbon nanotubes (CNTs), one-dimensional nanostructures, have attracted much attention since CNTs were discovered in 1991. Owing to their electrical conductance, chemical stability, high surface-to-volume ratio and fast electron transport properties, CNTs are widely used in the electroanalytical fields, including biomedical and environmental sensors. Moreover, the remarkable properties of CNTs in cooperation with metallic or semiconducting nanoparticles (NPs) suggest the possibility of developing novel electrochemical sensing devices for bio/chemical molecules. NPs-modified CNT electrode can allow an enhanced electrocatalytic activity and a promoted electron-transfer reactions, resulting in highly sensitive and fast-response sensing. In this dissertation, we present NPs/single-walled carbon nanotubes (SWCNTs)-based electrochemical sensing application in detail from preparation of patterned SWCNT electrodes to fabrication of NPs/SWCNTs hybrids and electrochemical detection of bio/chemical molecules. In Chapters 1 and 2, two different methods using O2 plasma and electrochemical reaction are introduced for the patterning of SWCNTs films on flexible, transparent plastic substrates. In Chapters 3-5, modification of various nanomaterials (e.g. Au, Pt, ZnO, and TiO2) on SWCNT electrodes is explored for electrochemical applications in bio/chemical molecules sensing (e.g. glucose, hydrogen peroxide, hydrazine and heavy metal). Particularly in Chapter 5, integration of NPs-modified SWCNT electrodes with microfluidic chip is demonstrated as a new attractive sensing platform with significant advantages derived from the combination of electrochemical analysis and microfluidic systems.