골 유도 재생을 위한 생체 활성 나노섬유의 개발

Abstract

Porosity and bioactivity of barrier membrane are key factors for Guided Bone Regeneration (GBR). In this study, we developed the bioactive nanofibers by immobilizing bone forming peptide (BFP1) which had no cytotoxicity and osteogenic differentiation potential on biocompatible poly(lactide-co-glycolic acid) (PLGA) nanofibers with a simple method for effective GBR. Polydopamine was coated on the electrospun nanofibers and BFP1 was immobilized on polydopamine coated nanofibers with weak base condition and simple dipping method. Immobilized BFP1 was then verified by characterizing surface chemical composition of nanofibers and quantified by flourescamine assay. We identified most compact distribution of ECM molecules containing collagen I on the surface of human mesenchymal stem cells (hMSCs) and broad cell spreading when hMSCs were cultured on bioactive nanofibers for 14 days by using fluorescene staining and SEM. The enhancement of ALP activities and calcium deposition in BFP1 immobilized nanofibers supported osteogenic differentiation potential. The group of bioactive nanofibers showed remarkable bone in-growth in the defect site by radiography and integration between the bioactive nanofibers and host bone by histology when the nanofibers were implanted onto mouse calvarial defect for 8 weeks. BFP1 immobilized nanofibers were successfully fabricated by polydopamine chemistry and the nanofibers enhanced osteogenic differentiation of hMSCs and bone regeneration of mouse cranium. The results suggest that our approach could be promising and applied clinically for GBR.