다중파형 고속 전압전류 스캔법 : 절대농도 절대농도 정량화 가능성에 가능성에 대한 고찰

Abstract

ABSTRACT

Multi-Waveform Fast-Scan Cyclic Voltammetry : Experimental study for absolute concentration quantification

Oh, Yoon Bae Dept. of Biomedical Engineering The Graduate School of Biomedical Science and Engineering Hanyang University

Neurotransmitters are endogenous chemicals that transmit signals between neurons and cell across a synapse. This neurochemical substance became significant biomarker by unraveling its neuro-functional role in brain along with electrophysiological signal. Many techniques have been proposed to measure neurotransmitter in brain in order to investigate brain function. Fast-Scan Cyclic Voltammetry(FSCV) is one of methods to measure neurotransmitters by detecting fine current occurring in brain by applying voltage through carbon fiber microelectrode(CFM). It is experimentally utilized to measure chemical fluctuation within mille seconds in mammals. Nonetheless, because the background signal in FSCV is relatively large and unstable, undistinguishable complex environment limit its application when it comes to clinical approach. Restrictive phasic change of neurotransmitter could be detectable by adopting background subtraction technique but its unstable background signal has fundamental limitation that we can’t measure absolute concentration. In this research, we propose Multi-waveform Fast-Scan Cyclic Voltammetry(Multi-waveform FSCV) based on adsorption, desorption reaction within CFM double layer to extract characteristic value of each solution. Theoretical simulation reaches a conclusion that differences each waveform has exponential decay pattern and experimental demonstration supports this hypothesis. We could estimate absolute amount of neurotransmitter by adopting multi-waveform FSCV using extracted characteristic value of basic buffer. Unlike conventional FSCV, we could analyze or measure additive element without background effect by not adopting unstable background subtraction method.