Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 이기정 | - |
dc.contributor.author | 박진우 | - |
dc.date.accessioned | 2020-03-07T16:36:19Z | - |
dc.date.available | 2020-03-07T16:36:19Z | - |
dc.date.issued | 2013-02 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/133595 | - |
dc.identifier.uri | http://hanyang.dcollection.net/common/orgView/200000421019 | en_US |
dc.description.abstract | In this thesis, novel vinylsulfone (VS) cyanine dyes (em. 550−850 nm) were designed and synthesized for fluorescent labeling of biomolecules via 1,2-addition reaction in aqueous conditions. Due to the virtue of chemical structures of both fluorophore and reactive group, these dyes could be significantly stable and reactive in various aqueous/organic conditions. A wide variety of pH, temperature, buffer concentration, and protein were tested for the optimal labeling condition. Also, novel cyanine dyes with a VS group were designed and synthesized for pH indication of biomolecules. Among the series of pH indicator candidates, a selected reactive dye and its labeled biomolecules showed a dramatic change of fluorescence intensity in a wide range of pH conditions. The pH indicating dye can be used to label protein molecules and maintains its optical properties when conjugated to a macromolecule, making it possible to observe pH distributions and changes in acidic and neutral pH conditions. Finally, as an application study using the VS cyanine dyes, we developed a wide ranged fluorescent molecular weight size marker (MWSM). These MWSMs covers upto near-infrared wavelengths, consisted of sequentially arranged VS cyanine dyes enabling proteomic analysis without post-stain for size indication. | - |
dc.publisher | 한양대학교 | - |
dc.title | The Development of Novel Cyanine Dyes with Vinylsulfone Group for the Optical Imaging | - |
dc.title.alternative | 광학 영상을 위한 신규의 비닐설폰계 시아닌 염료의 개발 | - |
dc.type | Theses | - |
dc.contributor.googleauthor | 박진우 | - |
dc.contributor.alternativeauthor | Park, Jin Woo | - |
dc.sector.campus | S | - |
dc.sector.daehak | 대학원 | - |
dc.sector.department | 화학공학과 | - |
dc.description.degree | Doctor | - |
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