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Methyl-[beta]-cyclodextrin inhibits cell growth and cell cycle arrest via a prostaglandin E(2) independent pathway

Title
Methyl-[beta]-cyclodextrin inhibits cell growth and cell cycle arrest via a prostaglandin E(2) independent pathway
Author
최한곤
Keywords
cyclin A; cyclin D1; cyclooxygenase-2; methyl-β-cyclodextrin
Issue Date
2004-02
Publisher
KOREAN SOC MED BIOCHEMISTRY MOLECULAR BIOLOGY
Citation
EXPERIMENTAL AND MOLECULAR MEDICINE, v. 36, No. 1, Page. 78-84
Abstract
Methyl-β-cyclodextrin, a cyclic oligosaccharide known for its interaction with the plasma membrane induces several events in cells including cell growth and anti-tumor activity. In this study, we have investigated the possible role of cyclooxygenase 2 (COX-2) in cell growth arrest induced by methyl-βcyclodextrin in Raw264.7 macrophage cells. Methylβ-cyclodextrin inhibited cell growth and arrested the cell cycle, and this cell cycle arrest reduced the population of cells in the S phase, and concomitantly reduced cyclin A and D expressions. Methyl-β-cyclodextrin in a dose- and time-dependent manner, also induced COX-2 expression, prostaglandin E2 (PGE2) synthesis, and COX-2 promoter activity. Pretreatment of cells with NS398,a COX-2 specific inhibitor completely blocked PGE2 synthesis induced by methyl-β-cyclodextrin, however inhibition on cell proliferation and cell cycle arrest was not effected, suggesting nonassociation of CO X-2 in the cell cycle arrest. These results suggest that methyl-β-cyclodextrin induced cell growth inhibition and cell cycle arrest in Raw264.7 cells may be mediated by cyclin A and D1 expression.
URI
https://search.proquest.com/docview/1800521876?accountid=11283https://repository.hanyang.ac.kr/handle/20.500.11754/132055
ISSN
1226-3613
DOI
10.1038/emm.2004.11
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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