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dc.contributor.author이영식-
dc.date.accessioned2020-03-02T06:23:17Z-
dc.date.available2020-03-02T06:23:17Z-
dc.date.issued2004-01-
dc.identifier.citationDEVELOPMENTAL BIOLOGY, v. 265, No. 2, Page. 491-501en_US
dc.identifier.issn0012-1606-
dc.identifier.issn1095-564X-
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0012160603006080?via%3Dihub#!-
dc.identifier.urihttps://repository.hanyang.ac.kr/handle/20.500.11754/132027-
dc.description.abstractNeurogenesis is one of the most complex events in embryonic development. However, little information is available regarding the molecular events that occur during neurogenesis. To identify regulatory genes and underlying mechanisms involved in the differentiation of embryonic stem (ES) cells to neurons, gene expression profiling was performed using cDNA microarrays. In mouse ES cells, we compared the gene expression of each differentiated cell stage using a five-stage lineage selection method. Of 10,368 genes, 1633 (16%) known regulatory genes were differentially expressed at least 2-fold or greater at one or more stages. At stage 3, during which ES cells differentiate into neural stem cells, modulation of nearly 1000 genes was observed. Most of transcription factors (Otx2, Ebf-3, Ptx3, Sox4, 13, 18, engrailed, Irx2, Pax8, and Lim3), signaling molecules (Wnt, TGF, and Shh family members), and extracellular matrix/adhesion molecules (collagens, MAPs, and NCAM) were up-regulated. However, some genes which may play important roles in maintaining the pluripotency of ES cells (Kruppel-like factor 2, 4, 5, 9, myeloblast oncogene like2, ZFP 57, and Esg-1) were down-regulated. The many genes identified with this approach that are modulated during neurogenesis will facilitate studies of the mechanisms underlying ES cell differentiation, neural induction, and neurogenesis.en_US
dc.description.sponsorshipThis work was supported by a grant from Korean Ministry of Health and Welfare (01-PJ10-PG8-01EC01-0023) to Y.-S.L.en_US
dc.language.isoen_USen_US
dc.publisherACADEMIC PRESS INCen_US
dc.subjectES cellsen_US
dc.subjectcDNA microarrayen_US
dc.subjectDifferentiationen_US
dc.subjectNeurogenesisen_US
dc.titleComprehensive transcriptome analysis of differentiation of embryonic stem cells into midbrain and hindbrain neuronsen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.ydbio.2003.09.041-
dc.relation.journalDEVELOPMENTAL BIOLOGY-
dc.contributor.googleauthorAhn, Joon-Ik-
dc.contributor.googleauthorLee, Ki-Hwan-
dc.contributor.googleauthorShin, Dong-Mi-
dc.contributor.googleauthorShim, Jae-Won-
dc.contributor.googleauthorLee, Jae-Seong-
dc.contributor.googleauthorChang, Sung Yeoul-
dc.contributor.googleauthorLee, Young-Seek-
dc.contributor.googleauthorBrownstein, Michael J-
dc.contributor.googleauthorLee, Sang-Hun-
dc.contributor.googleauthorLee, Yong-Sung-
dc.relation.code2009202536-
dc.sector.campusE-
dc.sector.daehakCOLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E]-
dc.sector.departmentDEPARTMENT OF MOLECULAR AND LIFE SCIENCE-
dc.identifier.pidyslee-


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