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Comprehensive transcriptome analysis of differentiation of embryonic stem cells into midbrain and hindbrain neurons

Title
Comprehensive transcriptome analysis of differentiation of embryonic stem cells into midbrain and hindbrain neurons
Author
이영식
Keywords
ES cells; cDNA microarray; Differentiation; Neurogenesis
Issue Date
2004-01
Publisher
ACADEMIC PRESS INC
Citation
DEVELOPMENTAL BIOLOGY, v. 265, No. 2, Page. 491-501
Abstract
Neurogenesis is one of the most complex events in embryonic development. However, little information is available regarding the molecular events that occur during neurogenesis. To identify regulatory genes and underlying mechanisms involved in the differentiation of embryonic stem (ES) cells to neurons, gene expression profiling was performed using cDNA microarrays. In mouse ES cells, we compared the gene expression of each differentiated cell stage using a five-stage lineage selection method. Of 10,368 genes, 1633 (16%) known regulatory genes were differentially expressed at least 2-fold or greater at one or more stages. At stage 3, during which ES cells differentiate into neural stem cells, modulation of nearly 1000 genes was observed. Most of transcription factors (Otx2, Ebf-3, Ptx3, Sox4, 13, 18, engrailed, Irx2, Pax8, and Lim3), signaling molecules (Wnt, TGF, and Shh family members), and extracellular matrix/adhesion molecules (collagens, MAPs, and NCAM) were up-regulated. However, some genes which may play important roles in maintaining the pluripotency of ES cells (Kruppel-like factor 2, 4, 5, 9, myeloblast oncogene like2, ZFP 57, and Esg-1) were down-regulated. The many genes identified with this approach that are modulated during neurogenesis will facilitate studies of the mechanisms underlying ES cell differentiation, neural induction, and neurogenesis.
URI
https://www.sciencedirect.com/science/article/pii/S0012160603006080?via%3Dihub#!https://repository.hanyang.ac.kr/handle/20.500.11754/132027
ISSN
0012-1606; 1095-564X
DOI
10.1016/j.ydbio.2003.09.041
Appears in Collections:
COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > MOLECULAR AND LIFE SCIENCE(분자생명과학과) > Articles
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