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Rh Blood Group Conversion using Engineered Nucleases

Title
Rh Blood Group Conversion using Engineered Nucleases
Author
김영훈
Advisor(s)
김형범
Issue Date
2014-08
Publisher
한양대학교
Degree
Master
Abstract
The Rh (Rhesus) blood group system (including the Rh factor) is the most important blood group system following ABO blood group. The Rh blood group system consists of mainly five antigens D, C, c, E, and e. The commonly used terms Rh factor, Rh positive and Rh negative refer to the D antigen only. In this study, we used TALENs (transcription activator-like effector nucleases), a powerful tool for genome editing on HiDEP-1 (human iPS cell-derived erythroid progenitor-1) cell line for the conversion of Rh positive patient cell line to Rh negative HiDEP-1 cell line. Additionally, we confirmed Rh negative HiDEP-1 cell line can differentiate into erythrocyte which does not express D antigen. Here, we established a technology to convert human blood groups using engineered nucleases. In addition, this technology could be further utilized for blood transfusion medicine.
URI
https://repository.hanyang.ac.kr/handle/20.500.11754/130008http://hanyang.dcollection.net/common/orgView/200000424785
Appears in Collections:
GRADUATE SCHOOL OF BIOMEDICAL SCIENCE AND ENGINEERING[S](의생명공학전문대학원) > BIOMEDICAL SCIENCE(의생명과학과) > Theses (Master)
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