교류 전기장을 이용한 나노 채널 내 DNA의 동특성 분석

Abstract

We have studied about dynamic characteristic of λ-DNA(48.5kbp) with AC electric field in the nanogrooves. To understand its characteristic, we prepared a device patterned nanogroove(80nm(h)ｘ100nm(w)) and microchannel(2µm(h) ｘ4µm(w)) on the silicon wafer at the first. The design for nano-/microchannel was optimized by using Finite Elements Method(FEM) and then the design was patterned on the silicon wafer from electron beam lithography, photo lithography and reactive ion etching process(R.I.E). The completed device was also bonded with 170um thick pyrex glass under enough temperature, voltage and force from which was called the anodic bonding process. We made an effort to establish whether the device showed good performance or not doing experiments such as current measure with electrometer and λ-DNA passing thru nanochannel. λ-DNA showed good performance in the nanochannel was analyzed by its stretched length to understand dynamic characteristic under a variety of range of voltage and frequency. Bulk range of frequency was chosen by an assumption that the natural frequency of λ-DNA would be calculated mathematically using theory of engineering vibration in mechanical engineering. The natural frequency of λ-DNA from theoretical calculation was about 35MHz. Voltage range was used until about 20Vp.p due to limitation of our own machine. At the low frequency from 1 to 50Hz, we figured it out that λ-DNA was stretched more under 5Vp.p and 10Hz than others. Also, at the high frequency, 300Hz, with over 2Vp.p, λ-DNA was collapsed. The result above showed that λ-DNA stretching length in nanochannel depended upon along change of frequency and voltage. Therefore, In this paper, we gave a challenge to understand dynamic characteristic of λ-DNA from relationship between voltage and frequency. In the future, our investigated results will help for DNA research, especially DNA stretching field, as well as be a method to measure mechanical properties of DNA using nanochannel.