Highly efficient neural differentiation of human ES cells on the feeder of differentiating NPC cells
- Title
- Highly efficient neural differentiation of human ES cells on the feeder of differentiating NPC cells
- Author
- 김진영
- Advisor(s)
- 이상훈
- Issue Date
- 2015-08
- Publisher
- 한양대학교
- Degree
- Master
- Abstract
- Human Embryonic stem cells (hESCs) and induced Pluripotent stem cells (iPSCs) can give as a powerful source for cell therapy to treat several degenerative disease such as Parkinson’s disease. Human neural precursor cells (hNPCs) derived from hESCs/iPSCs are major leading development of brain. It can be differentiated into neuronal population including astrocytes and oligodendrocytes. Many studies introduced the method for hESCs/iPSCs differentiation into NPCs. However, the efficiency depends on conditions as passage number of cells, hESCs/iPSCs line or specific RNA levels. Furthermore, cell transplantation surgery has effects forming teratoma using undifferentiated hESCs/iPSCs. To solve these problems, I introduced effective hNPCs differentiation method using hNPCs feeder layers. To eliminate expression of undifferentiation marker such as Oct4 or nanog, bFGF-free culture conditions with undifferentiation hESC mixed hNPCs were used for differentiation. This study can serve effective neural inducing without any possibility forming teramoma after cell transplantation for cell therapy.
- URI
- https://repository.hanyang.ac.kr/handle/20.500.11754/127808http://hanyang.dcollection.net/common/orgView/200000427017
- Appears in Collections:
- GRADUATE SCHOOL OF BIOMEDICAL SCIENCE AND ENGINEERING[S](의생명공학전문대학원) > BIOMEDICAL SCIENCE(의생명과학과) > Theses (Master)
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