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Highly efficient neural differentiation of human ES cells on the feeder of differentiating NPC cells

Title
Highly efficient neural differentiation of human ES cells on the feeder of differentiating NPC cells
Author
김진영
Advisor(s)
이상훈
Issue Date
2015-08
Publisher
한양대학교
Degree
Master
Abstract
Human Embryonic stem cells (hESCs) and induced Pluripotent stem cells (iPSCs) can give as a powerful source for cell therapy to treat several degenerative disease such as Parkinson’s disease. Human neural precursor cells (hNPCs) derived from hESCs/iPSCs are major leading development of brain. It can be differentiated into neuronal population including astrocytes and oligodendrocytes. Many studies introduced the method for hESCs/iPSCs differentiation into NPCs. However, the efficiency depends on conditions as passage number of cells, hESCs/iPSCs line or specific RNA levels. Furthermore, cell transplantation surgery has effects forming teratoma using undifferentiated hESCs/iPSCs. To solve these problems, I introduced effective hNPCs differentiation method using hNPCs feeder layers. To eliminate expression of undifferentiation marker such as Oct4 or nanog, bFGF-free culture conditions with undifferentiation hESC mixed hNPCs were used for differentiation. This study can serve effective neural inducing without any possibility forming teramoma after cell transplantation for cell therapy.
URI
https://repository.hanyang.ac.kr/handle/20.500.11754/127808http://hanyang.dcollection.net/common/orgView/200000427017
Appears in Collections:
GRADUATE SCHOOL OF BIOMEDICAL SCIENCE AND ENGINEERING[S](의생명공학전문대학원) > BIOMEDICAL SCIENCE(의생명과학과) > Theses (Master)
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