Combination therapy of HO-1 gene and Lipopolysaccharide binding peptide from HMGB1A in animal model of LPS-induced acute lung injury.
- Title
- Combination therapy of HO-1 gene and Lipopolysaccharide binding peptide from HMGB1A in animal model of LPS-induced acute lung injury.
- Other Titles
- 치료유전자와 HMGB1A의 LPS 결합 펩타이드를 이용한 급성폐손상의 복합적 치료.
- Author
- 김지연
- Alternative Author(s)
- 김지연
- Advisor(s)
- 이민형
- Issue Date
- 2016-02
- Publisher
- 한양대학교
- Degree
- Master
- Abstract
- Acute lung injury (ALI) is an inflammatory disease caused by lung infection, sepsis, trauma, aspiration, or ischemia. In this study, the combinational therapy with heme oxygenase-1 (HO-1) gene and lipopolysaccharide (LPS)-binding peptide (LBP) regions from high mobility group box-1 box A (HMGB1A) was evaluated for the treatment of ALI.
As a gene carrier, deoxycholic acid was conjugated to low molecular weight polyethylenimine (PEI, 1.8 kDa). Deoxycholic acid conjugated PEI (DA-3) was characterized as a carrier of the HO-1 gene in vitro. Gel retardation and heparin competition assays showed that DA-3 formed stable complex with DNA by electrostatic interaction. In the transfection and luciferase assay, DA-3 had higher transfection efficiency than high molecular weight PEI (PEI25k, 25kDa) and Lipofectamine in the L2 lung epithelial cells. These results were confirmed by the transfection assays with the HO-1 gene. In cytokine assays, the combined treatment with the HO-1 gene and LBP reduced the pro-inflammatory cytokines more efficiently that the single treatment of the HO-1 gene or LBP in the LPS activated macrophage cells. Therefore, the HO-1 gene and LBP may be useful for combination therapy for ALI.
- URI
- https://repository.hanyang.ac.kr/handle/20.500.11754/127194http://hanyang.dcollection.net/common/orgView/200000427953
- Appears in Collections:
- GRADUATE SCHOOL[S](대학원) > BIOENGINEERING(생명공학과) > Theses (Master)
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