기능성 멤브레인 플루이딕 소자를 이용한 아세트아미노펜의 전기화학적 측정

Abstract

아스코르브산의 존재 하에 아세트아미노펜의 측정이 가능한 기능성 멤브레인 플루이딕 소자를 제작하였다. 소자는 단일막 탄소나노튜브 전극, 나이트로셀룰로스 멤브레인 등의 소재들로 구성되어 있다. 나이트로셀룰로스 멤브레인에 보다 많은 음전하를 띠게 하기 위해 나피온을 이용하였고, 금나노입자와 폴리글루타믹애씨드를 단일막 탄소나노튜브 전극위에 증착하여 소자의 성능을 강화 하였다. 본 논문에서는 멤브레인 플루이딕 소자의 제작과 아세트아미노펜을 측정함으로서 소자의 성능을 평가하였다. 소자는 수직형태로 유체를 흐르게 하는 형태를 가지고 있어, 샘플 용액이 멤브레인을 수직으로 통과하여 흐르게 된다. 나피온을 사용한 나이트로셀룰로스 멤브레인과 금나노입자와 폴리글루타믹애씨드가 증착된 단일벽탄소 필름 전극을 사용함으로써 아스코르브산과 아세트아미노펜의 산화 피크를 분리할 수 있었다. 아세트아미노펜 산화 피크 전류 값은 50 μM부터 300 μM까지 농도에 대하여 선형성을 가졌으며 이는 약물 허용치 구간보다 넓은 범위이다. 또, 선형성의 결정계수는 r2 = 0.982이었다. 소자는 13.3 mA/M의 민감도를 가지고 있으며 측정한계는 15.0 μM이었다. 소자의 신호 값은 2주 동안 3.3%의 상대표준편차를 갖으며 안정적이었다. 재현성 또한 1.2에서 5.2%의 재현성을 보였다. 더 나아가 제작한 소자를 이용하여 의학용 샘플 속의 아세트아미노펜을 정량 할 수 있었다.|In the present study, we constructed a functional paper fluidic device and evaluated its electrochemical performance by analyzing acetaminophen in the presence of ascorbic acid. The device was composed of a single-walled carbon nanotube (SWCNT) electrode and nafion-modified nitrocellulose membrane. Negatively-charged nafion was employed to build up a more negative charge on the nitrocellulose membrane, and gold nanoparticles and poly glutamic acid (AuNP-PGA) were deposited on the SWCNT electrode to enhance the electrochemical performance of the device. The device had a vertical flow format in which the sample solution flowed vertically through the paper. Using the nafion-modified nitrocellulose membrane and AuNP-PGA/SWCNT film electrode as a component of the paper fluidic device, we obtained a distinguishable acetaminophen oxidation peak which was distinct from the ascorbic acid oxidation peak. The acetaminophen oxidation peak had a linear response with acetaminophen concentration, varying from 50 μM to 300 μM (r2 = 0.992), which was broader than the standard drug dose range. The device exhibited a sensitivity of 13.3 mA/M and a detection limit of 15.0 μM. The device was stable with a relative standard deviation of 3.3% (up to 2 weeks), and the reproducibility was 1.2 to 5.2%. Furthermore, the fabricated device accurately measured the amount of acetaminophen in pharmaceutical samples.; In the present study, we constructed a functional paper fluidic device and evaluated its electrochemical performance by analyzing acetaminophen in the presence of ascorbic acid. The device was composed of a single-walled carbon nanotube (SWCNT) electrode and nafion-modified nitrocellulose membrane. Negatively-charged nafion was employed to build up a more negative charge on the nitrocellulose membrane, and gold nanoparticles and poly glutamic acid (AuNP-PGA) were deposited on the SWCNT electrode to enhance the electrochemical performance of the device. The device had a vertical flow format in which the sample solution flowed vertically through the paper. Using the nafion-modified nitrocellulose membrane and AuNP-PGA/SWCNT film electrode as a component of the paper fluidic device, we obtained a distinguishable acetaminophen oxidation peak which was distinct from the ascorbic acid oxidation peak. The acetaminophen oxidation peak had a linear response with acetaminophen concentration, varying from 50 μM to 300 μM (r2 = 0.992), which was broader than the standard drug dose range. The device exhibited a sensitivity of 13.3 mA/M and a detection limit of 15.0 μM. The device was stable with a relative standard deviation of 3.3% (up to 2 weeks), and the reproducibility was 1.2 to 5.2%. Furthermore, the fabricated device accurately measured the amount of acetaminophen in pharmaceutical samples.